| 榄香烯抑制Raf/MEK/ERK信号通路诱导人源胶质瘤U87细胞凋亡 |
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| 引用本文： | 赵永顺,吴春明,董斌,位振清,徐英辉.榄香烯抑制Raf/MEK/ERK信号通路诱导人源胶质瘤U87细胞凋亡[J].实用药物与临床,2011,14(5). |
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| 作者姓名： | 赵永顺 吴春明 董斌 位振清 徐英辉 |
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| 作者单位： | 1. 大连医科大学,大连,116044
2. 大连医科大学附属第一医院神经外科,大连,116011 |
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| 摘 要： | 目的探讨莪术提取物榄香烯体外诱导胶质瘤细胞凋亡的机制。方法采用流式细胞术、West-ern印迹等方法,分别检测不同浓度榄香烯对人源U87胶质瘤细胞的凋亡诱导及其对U87细胞Raf-1、ERK、癌基因Bcl-2蛋白质表达的影响。结果榄香烯对人源U87胶质瘤细胞具有明显的凋亡诱导作用,该增殖抑制效应呈时间依赖性。榄香烯可明显下调U87细胞的磷酸化Raf-1、ERK、Bcl-2表达。结论体外榄香烯对胶质瘤细胞具有明显的凋亡诱导作用(呈时间依赖性),抑制Raf/MEK/ERK信号通路,从而下调其下游信号癌基因Bcl-2的表达,最终启动凋亡程序可能是榄香烯诱导U87细胞凋亡的机制。
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| 关 键 词： | 榄香烯 胶质瘤 Raf-1 ERK 细胞凋亡 |
Induction of elemene on apoptosis of U87 glioblastoma cells by inhibiting Raf/MEK/ERK pathway |
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| ZHAO Yong-shun,WU Chun-ming,DONG Bin,WEI Zhen-qing,XU Ying-hui.Induction of elemene on apoptosis of U87 glioblastoma cells by inhibiting Raf/MEK/ERK pathway[J].Practical Pharmacy and Clinical Remedies,2011,14(5). |
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| Authors: | ZHAO Yong-shun WU Chun-ming DONG Bin WEI Zhen-qing XU Ying-hui |
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| Institution: | ZHAO Yong-shun1,WU Chun-ming2,DONG Bin2,WEI Zhen-qing2,XU Ying-hui2(1.Dalian Medical University,Dalian 116044,China,2.Department of Neurosurgery,The First Affiliated Hospital of Dalian Medical University,Dalian 116011,China) |
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| Abstract: | Objective To study the mechanism by which elemene induces the apoptosis of human U87 glioblastoma cells in vitro.Methods Flow cytometry,Western blotting analysis w as used to test the effect of elemene on apoptosis of U87 glioblastoma cells,expression of Raf-1,ERK,Bcl-2 protein at different concentrations respectively.Results Elemene induced apoptosis of U87 glioblastoma cells in a dose-dependent manner.Elemene downregulated the expression of phosphorylated Raf-1,ERK and Bcl-2.Conclusion Elemene induced apo... |
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| Keywords: | Elemene Glioma Raf-1 Extracellular signal-regulated MAP kinases Apoptosis |
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