甲基化特异PCR方法对p16肿瘤抑制基因的研究

目的：研究肺癌、结肠癌组织中p16肿瘤抑制基因甲基化状态及其临床意义。方法：采用甲基化特异性聚合酶链反应（MSP）方法，特异扩增分析甲基化和非甲基化两种状态。结果：p16基因在人胚肺组织处于非甲基化状态；在人肺巨细胞癌细胞系（PLA801-D）呈甲基化状态；在检测的肿瘤标本中，60%肺癌组织及70%结肠癌组织出现甲基化状态。结论：p16基因的甲基化状态是肺癌、结肠癌组织中p16基因异常形式之一，有可能成为肿瘤分子诊断的重要标志，MSP方法有临床普及应用前景。

Study of p16 tumor suppressor gene with methylation specific PCR

Objective: To investigate the p16 tumor suppressor gene promoter ringer methylation status in human lung cancer、 colonic cancer. Methods:To take advantage of methylation-specific polymerase chain reaction (MSP) as a main method, the status of 5CpG island of p16 gene. was analysed. Results:p16 gene from normal lung tissue show only unmethy]ation status. The human pulmonary giant cell carcinoma cell line (PLA801-D) amplified only with the methylation primers of p16 gene. The positive rate of p16 gene methylation status in lung cancer and colonic cancer was 60%(6/10) and 70%(7/10), respectively.Conclusion:The methylation status of p16 gene in tumor tissues is one of the innormal type of p16 gene. The detecting of methylation patterns will be possible to become a novel biomarker in detecting early-stage cancer. MSP should prove extraordinarily valuable clinically.