顶空气相色谱法测定珍珠明目滴眼液中的冰片含量

目的改进珍珠明目滴眼液质量标准的检测方法。方法采用顶空进样毛细管色谱柱法,以SE-54毛细管柱(25m×320μm,0.6μm)为色谱柱,氮气为载气,FID为检测器测定。结果龙脑峰、异龙脑峰与内标峰完全分离,在0.89～5.91mg·mL-1浓度范围内,冰片与内标的峰面积比值(F)与冰片质量浓度(C)呈良好的线性关系,r=0.9982,平均加样回收率为95.88%,RSD=0.64%(n=9)。结论顶空法快速、灵敏、准确,可用于珍珠明目滴眼液中冰片含量的测定。

Determination of borneol in Zhenzhu Mingmu Eye Drops by Headspace GC

OBJECTIVE To improve the detection method of Zhenzhu Mingmu Eye Drops＇ quality standard. METHODS Take headspaee GC to determine, using SE--54 capillary column with FID detector, nitrogen as the carrier gas. RESULTS The peaks of （＋）- borneol, （-）- borneol and the internal standard were completely separated. Between the range of the concentration of 0. 89mg · mL^-1 and 5.91mg · mL^-1 ,there was a good linearity between the ratio of the area of borneol and internal standard and the concentration of borneol （ r =0. 9982）. The average recovery rate was 95.88% ,and RSD was 0. 64% （ n =9）. CONCLUSION The method of Headspace GC was quick,sensitive and accurate,which could be used in the content determination of borneol in the Zhenzhu Mingmu Eye Drops.