肺腺癌多药耐药细胞株特异表达的新基因cDNA片段在肺癌组织中表达的研究

目的　检测人肺腺癌多药耐药细胞株特异表达的新基因cDNA片段在多种肺癌组织及其相应癌旁正常组织中的表达情况。方法　分别运用原位杂交技术和Northernblot方法检测该基因cDNA片段在肺癌组织中的表达及定位情况。结果　组织原位杂交和Northernblot的检测结果均显示 ,在 12例肺腺癌组织中有 6例表达阳性 (5 0 % ) ,在其他肺癌组织及其癌旁正常组织中无表达 ,差异有统计学意义 (P <0 0 5或P <0 0 1)。图像分析显示 ,该基因在肺腺癌组织的相对表达量显著高于其癌旁正常组织 (P <0 0 5 )。结论　新基因片段在部分肺腺癌组织有表达 ,可能与临床上部分肺腺癌化疗效果不佳及MDR的产生有关。

Study on the expression of a novel cDNA fragments of a multidrug resistance gene from human lung adenocarcinoma cell line in lung cancer

Objective To study the expression of a new drug resistance-related gene cDNA fragment from human lung adenocarcinoma cell line in several types of lung cancer and their adjacent normal tissues. Methods The in situ hybridization histochemistry (ISHH) and Northern blot were employed to study the location and expression of the cDNA fragment in lung cancer cells. Results The results of ISHH and Northern blot showed that the expression of the fragment was discovered in 6 cases of 12 patients with lung adenocarcinoma, but not observed in the lung cancer tissues of other types and normal tissues(P<0.05, P<0.01). The result of image analysis of Northern blot showed that there was significant difference between lung adenocarcinoma tissues and normal lung tissues (P<0.01). Conclusion The expression of the new gene fragment exists in a portion of the lung adenocarcinoma tissues, which might help explain a poor response of a portion of adenocarcinomas to chemotherapy, thereby contributing to the MDR of tumors.