Comparison of evaporation techniques for the preparation of salivary cortisol for analysis by liquid chromatography-electrospray tandem mass spectrometry |
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| Authors: | Nelson Elizabeth A Palombo Enzo A Knowles Simon R |
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| Affiliation: | Environment and Biotechnology Centre, Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, VIC 3122, Australia. 4021975@student.swin.edu.au |
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| Abstract: | ObjectiveCompare evaporation techniques in the work-up procedure of saliva samples for cortisol analysis by a previously validated liquid chromatography–tandem mass spectrometry (LC–MS/MS) method.Design and methodsSamples were evaporated using freeze drying, centrifugal concentration, and nitrogen flow and analyzed by LC–MS/MS.ResultsCortisol concentrations detected using each technique did not significantly differ. Factors other than the recovery of cortisol were evaluated.ConclusionsEvaporation using freeze drying/centrifugal concentration is an automated process, reducing overall processing time for analysis of numerous samples. |
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