急性髓细胞性白血病细胞表面CD分子表达及临床意义研究

目的 探讨急性髓细胞性白血病(AML)细胞表面CD分子的表达情况.方法 采用CD45分子作为靶目标抗原,应用多参数流式细胞术检测300例AML患者细胞表面的CD分子,分析患者的免疫表型.结果以表达百分比﹥20%作为阳性,300例AML患者的CD抗原表达均不相同,cyt-MPO、CD33、CD117和CD38表达最强,为强阳性(80%~100%).以CD抗原表达>60%为阳性,结果显示:CD33、CD117、CD13、cyt-MPO四种抗原阳性表达的患者,阳性细胞所占的比例高于其他抗原,差异有统计学意义(P﹤0.05).CD15/CD64、CD15/CD33和CD33/CD64抗原的阳性表达比较,差异均有统计学意义(P﹤0.05);CD33/cyt-MPO抗原的阳性表达比较,差异无统计学意义(P﹥0.05).结论 AML细胞表面的CD抗原表达具有差异性,CD13、cyt-MPO、CD33、CD117呈高表达.CD分子在AML细胞中阳性表达的差异性可为治疗提供有价值的线索.

Expression of CD on the surface of acute myeloid leukemia cells and its clinical significance

Objective To investigate the expression of CD molecules on the surface of acute myeloid leukemia (AML) cells. Method Cell surface CD molecules of 300 AML patients were detected by multiparameter flow cytome-try using CD45 molecules as target antigens, to analyze the immunophenotype of the patients. Result Using expression percentage>20%as a positive cutoff value, the expression level varied among CD antigens in 300 AML patients, cyt-MPO、CD33、CD117 and CD38 were strong positive, with high expression level 80%~100%. When taking CD antigen expression percentage>60%as the positive cutoff value, the results showed that, the percentage of positive cells in pa-tients with positive expression of CD33, CD117, CD13 and cyt-MPO was higher than that in patients with positive ex-pression of other cells, and the differences were statistically significant (P<0.05). The differences between antigen CD15/CD64, CD15/CD33 or CD33/CD64 reached statistical significance (P<0.05). There was no significant difference in posi-tive expression of antigen found between CD33 and cyt-MPO (P>0.05). Conclusion The expression level varies among CD antigens on AML cell surface, while CD13, cyt-MPO, CD33 and CD117 were highly expressed. The difference of ex-pression levels of CD antigens on AML surface can provide valuable clues for treatment.