VEGF和p53蛋白在甲状腺肿瘤中的表达及在诊断甲状腺良、恶性肿瘤中的应用价值研究

目的 探讨血管内皮生长因子(VEGF)和p53蛋白在甲状腺肿瘤中的表达以及在良恶性肿瘤诊断中的价值.方法 选取甲状腺肿瘤患者100例,采用免疫组化技术检测组织中VEGF和p53蛋白的表达.结果 甲状腺恶性肿瘤VEGF和p53蛋白阳性表达率分别为62.90%和48.39%,明显高于甲状腺良性肿瘤(P<0.01);VEGF和p53蛋白在不同年龄、性别、TNM分期、病理类型及肿瘤直径的甲状腺恶性肿瘤患者中表达情况差异无统计学意义(P>0.05);甲状腺恶性肿瘤伴淋巴结转移患者VEGF和p53蛋白阳性表达率分别为93.10%和68.97%,明显高于不伴淋巴结转移患者(P<0.01);VEGF判断甲状腺恶性肿瘤灵敏度为62.90%,特异度为89.47%,阳性预测值为90.70%,阴性预测值为59.65%;p53蛋白判断甲状腺恶性肿瘤灵敏度为48.39%,特异度为84.21%,阳性预测值为83.33%,阴性预测值为50.00%;VEGF联合p53蛋白判断甲状腺恶性肿瘤灵敏度为70.97%,特异度为89.47%,阳性预测值为91.67%,阴性预测值为65.63%.结论 VEGF和p53蛋白表达上调可能与甲状腺恶性肿瘤淋巴结转移有关,同时在鉴别良恶性甲状腺肿瘤中有一定的应用价值.

The expression of VEGF and p53 protein in thyroid tumors and its value in the differential diagnosis of benign and malignant thyroid tumors

Objective To investigate the expression of vascular endothelial growth factor(VEGF) and p53 protein in thyroid tumors and its value in the differential diagnosis of benign and malignant tumors. Method 100 cases of pa-tients with thyroid tumors were selected, and the expression of VEGF and p53 protein in tissues were measured by immu-nohistochemistry. Result The positive expression rates of VEGF and p53 protein in malignant thyroid tumors were 62.90%and 48.39%, significantly higher than those in benign thyroid tumors (P<0.01). The expression of VEGF and p53 protein in patients with malignant thyroid cancer was not associated with age, gender, TNM stage, pathological type and tumor diameter (P>0.05). The positive expression rates of VEGF and p53 in malignant thyroid cancer patients with lymph node metastasis were 93.10% and 68.97%, significantly higher than those without lymph node metastasis (P<0.01). In the diagnosis of malignant thyroid tumor, VEGF had a sensitivity of 62.90%, a specificity of 89.47%, a positive predictive value of 90.70%and a negative predictive value of 59.65%;p53 protein had a sensitivity of 48.39%, a specifici-ty of 84.21%, a positive predictive value of 83.33% and a negative predictive value of 50.00%; The combination of VEGF and p53 had a sensitivity of 70.97%,a specificity of 89.47%,a positive predictive value of 91.67%,and a negative predictive value of 65.63%. Conclusion The upregulated of expression of VEGF and p53 protein may be associated with lymph node metastasis in thyroid cancer, and has some value in the differential diagnosis of benign and malignant thyroid tumors.