IL-17在NSCLC淋巴管形成和侵袭中的作用

目的 探讨白细胞介素-17(IL-17)在非小细胞肺癌(NSCLC)淋巴管形成和侵袭中的作用.方法 收集手术切除的NSCLC组织标本40例,采用免疫组织化学法检测NSCLC组织中IL-17表达情况及淋巴管密度;采用MTT实验检测IL-17对淋巴管内皮细胞(LEC)增殖的影响;采用Transwell实验和淋巴管形成实验检测IL-17对LEC细胞迁移和管样结构形成的影响.结果 IL-17蛋白阳性表达患者的淋巴管密度高于IL-17蛋白阴性表达患者(P﹤0.05);0、0.5、1.0、5.0和10.0 ng/ml IL-17对LEC增殖活力的影响比较,差异无统计学意义(P﹥0.05);对照组和IL-17组透膜细胞数比较,差异无统计学意义(P﹥0.05);LLC组透膜细胞数为(58.70±12.52)个,高于对照组和IL-17组(P﹤0.05);LLC+IL-17组透膜细胞数最高,为(98.82±20.16)个,高于对照组、IL-17组和LLC组(P﹤0.05);对照组和IL-17组管样结构分支数比较,差异无统计学意义(P﹥0.05);LLC组管样结构分支数高于对照组和IL-17组(P﹤0.05);LLC+IL-17组管样结构分支数最高,为(155.82±29.92)个,高于对照组、IL-17组和LLC组(P﹤0.05).结论 IL-17对LEC的增殖、迁移和管样结构形成无直接的促进作用,但可通过对NSCLC细胞的作用间接促进LEC的侵袭和转移.

Role of IL-17 in the formation and invasion of lymphatic vessels in NSCLC

Objective To investigate the role of interleukin-17 (IL-17) in the formation and invasion of lymphatic vessels in non small cell lung cancer (NSCLC). Method 40 cases of NSCLC specimens were collected, in which the ex-pression of IL-17 and lymphatic vessel density were detected by immunohistochemistry;the effect of IL-17 on the prolif-eration of lymphatic endothelial cells (LEC) was detected by MTT assay, and the effect of IL-17 on migration and tube like structure formation of LEC cells was detected by Transwell assay and lymphatic vessel formation assay. Result The lymphatic vessel density in patients with positive expression of IL-17 was significantly higher than that in those with neg-ative expression of IL-17 (P<0.05);IL-17 at 0, 0.5, 1.0, 5.0 and 10.0 ng/ml had similar effect on the proliferation activity of LEC cells (P>0.05);there was no significant difference in transmembrane cell number between control group and IL-17 group (P>0.05);the number of transmembrane cells in LLC group was (58.70±12.52), which was significantly higher than that in control group and IL-17 group (P<0.05); the number of transmembrane cells in LLC+IL-17 group was (98.82 ± 20.16), which was significantly higher than that in other groups (P<0.05);there was no significant difference in the number of tube like structures between control group and IL-17 group (P>0.05);the number of tube like structures in LLC group was significantly higher than that in control group and IL-17 group (P<0.05), and was the highest in LLC+IL-17 group at (155.82±29.92) compared with other groups (P<0.05). Conclusion IL-17 does not directly influence the pro-liferation, migration and tube like structure formation of LEC, but it can promote the lymph node metastasis of the cell by action on NSCLC cells.