mTOR和survivin在神经胶质瘤中的表达水平及临床意义

目的 探讨哺乳动物雷帕霉素靶蛋白(mTOR)和生存素(survivin)在神经胶质瘤中的表达水平及临床意义.方法 选取89例神经胶质瘤患者(观察组)和10例因脑外伤须行脑减压术的患者(对照组),观察组根据病理分级分为低分级组(Ⅰ级20例、Ⅱ级24例)和高分级组(Ⅲ级21例、Ⅳ级24例).取观察组患者的神经胶质瘤组织标本和对照组患者的正常脑组织标本,通过免疫组化法检测mTOR和survivin的表达水平,并分析其与临床特征的关系.结果 mTOR和survivin在对照组标本中均未有阳性表达,而观察组中高分级组患者的mTOR和survivin阳性率高于低分级组(P﹤0.05);mTOR和survivin的表达水平均与病理分级呈正相关(r=0.443,P=0.000;r=0.379,P=0.000).mTOR和survivin在不同病理分级、肿瘤大小患者中的表达情况比较,差异有统计学意义(P﹤0.05),且survivin在不同病理类型患者中的表达情况比较,差异有统计学意义(P﹤0.05).mTOR和survivin在神经胶质瘤中的表达呈正相关(r=0.279,P﹤0.05).结论 mTOR和survivin在神经胶质瘤中的表达明显增加,随着神经胶质瘤病理分级的升高,其表达水平升高且两者呈正相关.mTOR和survivin可能在神经胶质瘤的发展过程中发挥着重要作用,可为临床靶向治疗提供指导.

Expression and clinical significance of mTOR and survivin in gliomas

Objective To study the expression level and clinical significance of mTOR and survivin in human glio-ma. Method 89 cases of glioma (study group) and 10 cases of cerebral decompression due to traumatic brain injury (con-trol group) were included in the study;according to the pathological examination results of gliomas, 89 patients in study group were stratified as lower grade group (grade I:20 cases, grade II:24 cases) and higher grade group (grade III:21 cas-es, grade IV:24 cases);the specimens of glioma of study group and normal brain tissues of control group were collected and detected by immunohistochemistry for mTOR and surviving, and the correlation with clinical features were investigat-ed. Result No positive expression of mTOR and survivin were observed in the control group, while those in higher grade group were higher than that in the lower grade group (P<0.05);a positive correlation between pathological grading and expression of mTOR and survivin were observed (r=0.443, P=0.000; r=0.379, P=0.000); besides, mTOR and sur-vivin expression were varied in patients with different pathological grade and tumor size (P<0.05), while survivin level was also associated with pathological types (P<0.05);mTOR and survivin were positively correlated with each other in gliomas (r=0.279, P<0.05). Conclusion mTOR and survivin expression are markedly enhanced in gliomas, and are in-creased as pathological grade elevates, indicating a key role in the development of glioma, which may provide scientific guidance for clinical targeted therapy.