七氟烷对血管性认知功能损害大鼠炎性反应的影响

目的 探讨七氟烷对血管性认知功能损害大鼠炎性反应的影响.方法 成年雌性Wistar大鼠42只,体重200～250 g,随机分为3组:假手术组(S组,n=6)、血管性认知功能损害组(VCI组,n=18)和七氟烷组(Sev组,n=18).采用永久结扎大鼠双侧颈总动脉法建立血管性认知功能损害模型.Sev组于双侧颈总动脉结扎后,持续吸入七氟烷(呼气末浓度1.8%)和氧气0.5 L/min 12 h.S组于干预结束后2 d,VCI组和Sev组于干预结束后2、14、26 d时进行水迷宫实验和穿梭箱实验,记录学习、记忆潜伏期和被动、主动逃避次数.S组于干预结束后12 d,VCI组和Sev组于吸入七氟烷后12、24、36 d时各取6只大鼠,心脏取血后处死,取皮质和海马组织,采用酶联免疫吸附法测定血清和脑组织白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的水平,采用RT-PCR法测定脑组织IL-1β mRNA和TNF-α mRNA的表达.结果 与S组比较,VCI组和Sev组学习潜伏期延长,记忆潜伏期、被动和主动逃避次数降低,脑组织和血清IL-1β和TNF-α的水平升高,脑组织IL-1β mRNA和TNF-αmRNA的表达上调(P<0.01);与VCI组比较,Sev组学习潜伏期缩短,记忆潜伏期、被动和主动逃避次数增加,脑组织和血清IL-1β和TNF-α的水平降低,脑组织IL-1β mRNA和TNF-α mRNA的表达下调(P<0.01).结论 七氟烷可通过抑制炎性反应减轻大鼠血管性认知功能损害.

Effects of sevoflurane on inflammatory reaction in rats with vascular cognitive impairment

Objective To investigate the effects of sevoflurane on inflammatory reaction in rats with vascular cognitive impairment. Methods Forty-two adult female Wistar rats, weighting 200-250 g, were randomly divided into 3 groups: sham operation group(group S, n = 6), vascular cognitive impairment group (group VCI, n = 18) and sevoflurane group (group Sev, n = 18). Vaesular cognitive impairment was induced by permanent bilateral occlusion of both common carotid arteries. Group Sev received inhalation of sevnflurane (end-tidal concentration 1.8%) in oxygen 0.5 L/min for 12 h after both common carotid arteries were occluded. Learning ability and memory were assessed using Morris water maze test and shuttle box task 2 d after treatment in group S while 2, 14, 26 d after treatment in group VCI and Sev. The level of IL-1β and TNF-α in serum and brain tissue were determined by ELISA and the expression of IL-1β mRNA and TNF-α mRNA in brain tissue were determined by RT-PCR 2 d after treatment in group S while 12, 24, 36 d after treatment in group VCI and Sev. Results Compared with group S, the latency of study was significantly prolonged, the latency of memory, the number of times of passive avoidance response and active avoidance response were significantly decreased, the level of IL-1β and TNF-α in serum and brain tissue were significantly increased, the expression of IL-1β mRNA and TNF-α mRNA in brain tissue were significantly up-regulated in group VCI and Sev. Compared with group VCI, the latency of study was significantly shortened, the latency of memory, the number of times of passive avoidance response and active avoidance response were significantly increased, the level of IL-1β and TNF-α in serum and brain tissue were significantly decreased, the expression of IL-1β mRNA and TNF-α mRNA in brain tissue were significantly down-regulated in group Sev. Conclusion Sevoflurane can attenuate vascular cognitive impairment in rats by inhibiting inflammatory reaction.