| 反相高效液相色谱法同时测定荨麻中绿原酸和咖啡酸 |
| |
| 引用本文： | 李小平,张菁华.反相高效液相色谱法同时测定荨麻中绿原酸和咖啡酸[J].中国医药导报,2012(31):112-114. |
| |
| 作者姓名： | 李小平 张菁华 |
| |
| 作者单位： | [1]上海中医药大学中药学院,上海201203 [2]江苏畜牧兽医职业技术学院,江苏泰州225300 |
| |
| 摘 要： | 目的建立荨麻中绿原酸和咖啡酸含量测定的方法。方法色谱柱:Betasil C18(150 mm×4.6 mm,5μm),流动相为甲醇(A)-0.5%三氟乙酸(B)梯度洗脱,流速:1.0 mL/min,柱温:30℃,检测波长:323 nm。结果分别在4.8～96.0μg/mL和0.23～4.60μg/mL范围内绿原酸和咖啡酸呈良好的线性关系,方法回收率分别为100.5%、98.1%。结论该方法简便、快速、准确,可用于荨麻质量的控制。
|
| 关 键 词： | 荨麻 绿原酸 咖啡酸 RP-HPLC |
RP-HPLC determination of chlorogenic acid and caffeic acid in Urtica fissa E. Pritz. |
| |
| LI Xiaoping,ZHANG Jinghua.RP-HPLC determination of chlorogenic acid and caffeic acid in Urtica fissa E. Pritz.[J].China Medical Herald,2012(31):112-114. |
| |
| Authors: | LI Xiaoping ZHANG Jinghua |
| |
| Institution: | 1.School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.Jiangsu Animal Husbandry and Veterinary College,Jiangsu Province,Taizhou 225300,China |
| |
| Abstract: | Objective To develop the HPLC method to determine the proportion of chlorogenic acid and caffeic acid in Urtica fissa E.Pritz..Methods Betasil C18（150 mm×4.6 mm,5 μm） was used,the mobile phase was acetonitrile-0.5%THF with gradient elution at the flow rate of 1.0 mL/min,the column and temperature was 30℃,the absorbance was monitored at 323 nm.Results The linear range was 4.8-96.0 μg/mL for chlorogenic acid and 0.23-4.60 μg/mL for caffeic acid.The average recoveries（n = 6） of chlorogenic acid and caffeic acid were 100.5% and 98.1%,respectively.Conclusion The method is found to be simple and accurate for quality control of Urtica fissa E.Pritz.. |
| |
| Keywords: | Urtica fissa E Pritz Chlorogenic acid Caffeic acid RP-HPLC |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
