小儿细菌性肺炎支气管肺泡灌洗液IL-6及IL-8变化的探讨

目的 探讨支气管肺泡灌洗液(BALF)中白细胞介素(IL)-6、IL-8与小儿细菌性肺炎的相关性.方法 采用双抗体夹心ELISA法分别测定26例细菌性肺炎患儿(肺炎组)治疗前后和14例无明显气道炎性反应患儿(对照组)BALF中IL-6、IL-8水平,并行中性粒细胞(PMN)和肺泡巨噬细胞(AMs)计数.结果 肺炎组治疗前BALF中IL-6和IL-8水平、细胞总数、PMN明显比治疗后及对照组高(P<0.01).革兰阴性细菌感染患儿BALF中IL-6、IL-8水平比革兰阳性细菌感染显著升高(P<0.01).肺炎组BALF中IL-6水平与AMs呈显著正相关(r=0.7615.P<0.01),肺炎组BALF中IL-8水平分别与PMN和AMs呈显著正相关(r=0.8956,r=0.6018,P<0.01).结论 IL-6、IL-8参与小儿细菌性肺炎的炎性反应,BALF中IL-6和IL-8水平动态变化对评价小儿细菌性肺炎的病情有一定的临床意义.

Dynamic changes of bronchoalveolar lavage fluid levels of IL-6 and IL-8 in child bacterial pneumonia

Objective To investigate the relationship between bronchoalveolar lavage fluid (BALF) levels of interleukin (IL)-6, IL-8 and child bacterial pneumonia. Methods BALF levels of IL-6, IL-8 were detected by enzyme linked immunosorbent assay (ELISA) in 26 patients with bacterial pneumonia and 14 controls. Neutrophils and alveolar macrophages (AMs) of BALF were examined. Results BALF levels of IL-6, IL-8, total cell count and neutrophils of child bacterial pneumonia before treatment were significantly higher than those of pneumonia after treatment and controls (P<0.01). BALF levels of IL-6, IL-8 were significantly higher in Gram-negative infection than those in Gram-positive infection (P<0.01). BALF level of IL-6 showed positive correlation with AMs in pneumonia (r=0.7615, P<0.01). BALF level of IL-8 was correlated positively with neutrophils and AMs respectively in pneumonia (r=0.8956, r=0.6018, P<0.01). Conclusions IL-6 and IL-8 play the roles in the development of airway inflammation in child bacterial pneumonia. Detection of BALF levels of IL-6 and IL-8 is valuable to predict the state of child bacterial pneumonia.