神经生长因子对谷氨酸介导的基底核神经元损伤的保护作用

本研究的目的是观察神经生长因子(nerve growth factor,NGF)对谷氨酸诱导的基底核神经元损伤的保护作用。取出生后1d乳鼠前脑基底核神经元培养,随机分为正常对照组、模型组(谷氨酸损伤组)和NGF保护组。用倒置相差显微镜进行活细胞观察,采用RT-PCR技术检测前脑基底核神经元的神经生长相关蛋白-43(growth associated protein-43,GAP-43)的表达。结果显示谷氨酸损伤组神经元胞体回缩,突起消失或断裂。NGF保护组的神经元绝大多数胞体饱满,突起明显,细胞间的网络联系仍清晰可见,接近于正常对照组;NGF保护组神经元内GAP-43mRNA表达比谷氨酸损伤组高,两者比较有统计学意义(P<0.05)。结果提示,NGF能保护基底核神经元免受谷氨酸兴奋性毒性的损伤。

Protective effects of NGF on basal ganglia neuron injury induced by glutamate

To investigate the protective effects of nerve growth factor (NGF) on primary cultured basal ganglia neurons from glutamate-induced damage. Basal ganglia cultures were made from rats at postnatal day 1. The slices were divided into 3 groups: control group, model group (glutamate group) and NGF group. The percentage of living basal ganglia neurons was determined under inverted phase contrast microscope. The GAP-43 mRNA expression was detected by using RT-PCR method. The basal ganglia neurons treated with glutamate swelled or shrunk and the processes broke, became shorter or even disappeared after incubation with glutamate. While the basal ganglia neurons in NGF group kept their cell shapes with prominent processes just similar to those of normal cells. In the cell culture, NGF mainly increased the number of basal ganglia neurons and promoted the development of the processes. The effects of NGF on the number and morphology of basal ganglia neurons was obviously superior to those of glutamate group. The quantity of GAP-43 mRNA in NGF group was higher than that in the glutamate group (P<0.05). NGF can protect basal ganglia neurons from the injury by glutamate.