集成毛细管电泳芯片分离尿蛋白条件的初步优化

目的:探讨集成毛细管电泳芯片快速分离尿蛋白条件的初步优化。方法:考察了分离电压(1200～2000V)、进样时间(10～30s)及乙胺浓度(0.5～2%(v/v))对尿蛋白分离的影响。结果:以75mmol/L pH10.3硼酸盐缓冲液含9.73μmol/L乳酸钙、1%(v/v)乙胺为电泳缓冲液,在以500V的进样电压进样15s、分离电压1500V条件下,电泳分离尿蛋白能分出更多蛋白峰。结论:通过优化可以有效地提高电泳芯片分离尿蛋白的效果。

Initial optimization of integrated capillary electrophoresis chip separation of urine proteins

Objective:To investigate the initial optimization of integrated capillary electrophoresis chip for rapid separation of urine proteins. Methods:Separating voltage(1 200～2 000V), injection time(10～30s) and ethylamine concentration(0.5～2% (v/v))were integratively evaluated to select the optimal condition of separating urine proteins. Results:The electrophoresis was finished in 4 minutes. Results were obained through comprehensive analysis: more resolved protein peaks could be achieved by using 15s injection time with 500V, 1500V separation voltage and 75mmol/L pH10.3 borate buffer containing 9.73μmol/L calcium lactate and 1%(v/v) ethylamine as electrophoresis buffer. Conclusion:Elevated efficiency of separating urine proteins using integrated capillary electrophoresis chip can be gained by optimization of electrophoresis conditions.