细胞周期抑制蛋白p27kip1和周期蛋白表达与大肠癌发生预后

目的探讨细胞周期调控抑制因子p27kip1蛋白和周期蛋白E(cyclinE)在大肠腺癌发生发展中的作用。方法采用免疫组化技术SP法,检测p27kip1和cyclinE蛋白在37例大肠腺癌和26例正常大肠黏膜组织中的表达。结果p27kip1蛋白阳性表达率在大肠腺癌组织中为45.9%,显著低于正常大肠黏膜组织(χ2=4.590,P<0.05),并与大肠腺癌组织分化程度和淋巴结转移均有关(χ2=9.653,χ2=6.838,P<0.05);cyclinE蛋白阳性表达率在大肠腺癌组织中为64.9%,显著高于正常大肠黏膜组织(χ2=4.285,P<0.05),并与大肠腺癌组织分化程度和淋巴结转移均有关(χ2=8.397,χ2=5.261,P<0.05);p27kip1蛋白阳性表达组cyclinE蛋白阳性表达率低于p27kip1蛋白阴性表达组,但两者无相关(r=-0.1133,P>0.05)。结论p27kip1蛋白的低表达和/或cyclinE蛋白的高表达在大肠腺癌发生中发挥重要作用,并可能有助于预测其恶性程度及淋巴结转移,可能对大肠腺癌的治疗和生活质量的改善有指导作用。

Expression of inhibiting protein of cell cycle-p27kip1 and cyclinE proteins and their relationships with the genesis and prognosis of human colorectal adenocarcinoma

Aim The relationships between p27kip1 protein as negative factor of cell cycle regulation and colorectal adenocarcinoma have been seldomly reported in China.This article aims to investigate the effects of inhibiting factor of cell cycle regulation-p27kip1 and cyclinE proteins in the genesis and progression of human colorectal adenocarcinoma.Methods The expressions of p27kip1 and cyclinE proteins in tumor tissues of 37 patients with colorectal adenocarcinoma and in normal colorectal mucosal tissues of 26 subjects were detected by SP immunohistochemical technique.Results p27kip1 protein positive-expression rate in tumor tissues of colorectal adenocarcinoma was 45.9％ , which was lower than that in normal colorectal mucosal tissues (χ 2=4.590,P< 0.05)， p27kip1 protein positive-expression correlated significantly with tumor cell differentiation and lymph node metastasis (χ 2=9.653;χ 2=6.838,P< 0.05)； cyclinE positive-expression rate in tumor tissues was 64.9％， which was higher than that in normal colorectal mucosal tissues (χ 2=4.285,P< 0.05)； cyclinE positive-expression also correlated significantly with tumor cell differentiation and lymph node metastasis (χ 2=8.397;χ 2=5.261,P< 0.05); cyclinE protein positive-expression rate in the tumor tissues of p27kip1 protein positive-expression group was lower than that in p27kip1 protein negative-expression group, there were no significantly correlation between the two groups ( r =－ 0.1133, P >0.05).Conclusion The decreased expression of p27kip1 protein and/or over－ expression of cyclinE protein may play an important role in the genesis of colorectal adenocarcinoma and might be helpful to the determination of malignant degree and lymph node metastasis as well as the treatment and living quality of the diseases.