食管癌高发区食管鳞状细胞癌组织nm23-H_1蛋白的表达

目的:通过对食管癌组织中肿瘤转移抑制基因nm23变化的研究,进一步了解食管癌转移发生的分子学基础。方法:采用免疫组织化学(ABC)方法,对食管癌高发区68例手术切除的食管癌组织中nm 23-H_1蛋白聚集进行了研究。结果:68例手术标本病理检查均为食管鳞状细胞癌,其中38例出现淋巴结鳞癌转移病灶。研究表明:nm 23-H_1免疫染色与食管鳞癌的分化程度、PCNA标记指数和浸润程度无关,但与食管鳞癌淋巴结转移有关,nm 23-H_1低表达组淋巴结转移率(70.27％)明显高于过表达组的转移率(38.71％),P<0.05。nm23-H_1表达存在有异质性。结论:nm 23-H_1低表达在食管鳞癌的转移中起一定的作用。

Expression of nin23-H1 protein in human esophageal squamous cell carcinoma in high incidence area of esophageal cancer in China

To investigate the changes of tumor metastatic suppressor gene nm23 in esophageal cancers, and further to understand the molecular basis of carcinogenesis in esophageal cancers.Methods: Immunohistochemistry method (ABC) was used to determine the immunoreactivity of nm23-H1 in 68 case collected from patient with primary and metastatic esophageal cancer in Linzhou (Linxian) city of China, a high incidence area for esophageal carcinoma. Results: Histopathologically, the 68 cases were found with esophageal squamous cell carcinoma (SCC), of which, 38 cases were found with lymph node metastasis .The immunostaining of nm23-H1 protein have no correlation with differentiation,PCNA labelling index and invasion in esophageal SCC.The low expression of nm23-H1 protein have correlation with the metastatic rate of esophageal SCC, the metastatic rate of low expression of nm23-H1 protein (70.27%) were higher than those with over expression of nm23-H1 protein (38.71 %),P< 0.05.The immunoreactivity of nm23-H1 protein in esophageal SCC have heterogeneity. Conclusions: The present study suggested that the low expression of nm23-H1 protein might play a significant role in metastasis of esophageal cancer.