益生菌对大鼠回肠造口模型肠道菌群和并发症的作用

目的探讨益生菌制剂对大鼠回肠造口术后肠道功能恢复和造口周围刺激性皮炎的影响。方法取周龄相似(10~13周)体重相近(340~370 g)的无特定病原体(SPF)级雄性SD大鼠18只采用随机数字表法将其平均分为嗜酸乳杆菌组、布拉式酵母菌组和对照组,每组6只,行回肠造口术。分别予嗜酸乳杆菌、布拉式酵母菌及常规饮食不同干预。于第10天评估血清前白蛋白(PA)和白蛋白(ALB)、小肠推进率、造口周围刺激性皮炎(DET)和粪便布里斯托(Bristol)评分、肿瘤坏死因子-α,并比较3组间菌群差异。计量资料组间比较采用单因素方差分析,而进一步两两比较采用LSD分析。结果术后第10天布拉氏酵母菌组和嗜酸乳杆菌组体重、PA、ALB均高于对照组(287.67±20.00)g比(263.67±18.25)g比(231.00±19.56)g、(194.27±6.76)mg/L比(186.00±9.23)mg/L比(166.09±3.77)mg/L、(20.14±2.06)g/L比(19.81±1.91)g/L比(15.67±1.17)g/L],差异均有统计学意义(F=13.051、26.013、11.987,P<0.01);小肠推进率、DET、Bristol评分和肿瘤坏死因子-α水平均低于对照组(36.16±7.93)%比(42.18±3.73)%比(46.08±5.38)%、(4.50±1.05)比(5.00±1.10)比(7.33±0.82)、(4.45±0.19)比(4.60±0.19)比(5.52±0.26)、(58.28±20.85)ng/L比(51.00±18.10)ng/L比(99.44±41.90)ng/L],差异均有统计学意义(F=4.248、13.876、43.382、4.883,P<0.05)。线性判别分析效应量(LefSe)分析显示,益生菌组的梭菌属-产气荚膜梭菌的含量明显低于对照组(线性判别分析值=3,P<0.05),差异有统计学意义。结论益生菌制剂有助于促进回肠造口术后肠道功能恢复,维持肠道菌群稳态,减少并发症发生。

Effects of probiotics on intestinal microbiota and complications in rat ileostomy model

Objective To investigate the effects of probiotics on intestinal function recovery and irritant dermatitis around ileostomy in rats after ileostomy.Methods A total of 182、sprague dawley(SD)rats with the similar age and weight provided by Beijing Huafukang Biotechnology were randomly divided into lactobacillus acidophilus group,S.boulardii group and control group.Each group was performed ileostomy and given the corresponding diet.Serum prealbumin(PA),albumin(ALB),small intestinal propulsive rate,colostomy dermatitis score and Bristol Score,and tumor necrosis factor were evaluated on the 10th day.The bacterial difference was compared.One-way ANOVA and least signficant difference(LSD)were used for statistical analysis.Results On the 10th day after surgery,the body weight,PA and ALB in the boulardii group and the lactobacillus acidophilus group were significantly increased as compared with those in the control group(287.67±20.00)g vs.(263.67±18.25)g vs.(231.00±19.56)g,(194.27±6.76)mg/L vs.(186.00±9.23)mg/L vs.(166.09±3.77)mg/L,(20.14±2.06)g/L vs.(19.81±1.91)g/L vs.(15.67±1.17)g/L]with the differences being statistically significant(F=13.051,26.013,11.987,P<0.01);small bowel advancement rate,discoloration-erosion-tissue score(DET)score,Bristol score and tumor necrosis factor-αlevels were lower than those in the control group(36.16±7.93)%vs.(42.18±3.73)%vs.(46.08±5.38)%,(4.50±1.05)vs.(5.00±1.10)vs.(7.33±0.82),(4.45±0.19)vs.(4.60±0.19)vs.(5.52±0.26),(58.28±20.85)ng/L vs.(51.00±18.10)ng/L vs.(99.44±41.90)ng/L],with the differences being statistically significant(F=4.248,13.876,43.382,4.883,P<0.05).LefSe analysis showed that the contents of clostridium and clostridium-perfringens in the probiotics group were significantly reducedlinear discriminant analysis(LDA)=3,P<0.05].Conclusion Probiotics can promote the recovery of intestinal function after ileostomy,maintain microbial homeostasis,and reduce the occurrence of complications.