结肠切除吻合术大鼠动物模型的建立

目的构建结肠切除吻合术大鼠动物模型。方法2019年9月至2019年12月将12只Sprague-Dawley大鼠通过随机数表随机分为两组:对照组随机取3只,仅做剖腹探查;实验组9只,从结肠无血管区离断肠管并进行缝合。记录手术时间,术后监测体重变化。于术后第7天进行肠道造影观察肠管吻合口愈合情况。于第8天行剖腹探查大鼠腹腔内部形态学变化,测量吻合口爆破压力,并通过采集吻合口及周围组织,进一步观察吻合口组织学的变化,计量资料采用t检验。结果该模型构建平均时间为22 min。大鼠的存活率为89%。造影显示肠管通畅,无梗阻。剖腹探查,肉眼可见吻合口存在轻微粘连,粘连部分可分离,吻合口周围有血管组织形成。与对照组比较,实验组苏木精-伊红染色可见吻合口有新生肉芽组织形成,并有小血管生成,未发现结肠上皮、大肠腺的存在。马松染色可见胶原纤维沉积。结论通过该方法可成功构建结肠切除吻合术大鼠动物模型。

Establishment of rat model of colectomy and anastomosis

Objective To establish the rats model of colectomy and anastomosis.Methods From September 2019 to December 2019,12 male Sprague-Dawley rats provided by the Comparative Medicine Department of the General Hospital of the Eastern Theater Command were divided into two groups randomly:experimental group and control group.There were 9 sprague-dawley(SD)rats in the experimental group,and the colon was cut and sutured.The patients in the control group only underwent exploratory laparotomy.The operation time was recorded and body weight changes were monitored.X-ray examination was done to observe the conditions of intestinal anastomosis at the 7th day after the operation.At the 8th day,the laparotomy was performed again to investigate the morphological changes inside the rat abdominal cavity.The burst pressure of anastomotic stoma was measured.The histological changes of the anastomosis were further detected by collecting the anastomotic and surrounding tissues.T test was used for counting data.Results The average operation time was 22 min and the survival rate was 89%.The X-ray examination revealed the intestinal canal had no obstruction.The results of laparotomy demonstrated there was slight adhesion at the anastomotic site and the adhesion could be separated.The formation of vascularized tissue blood vessel could be found.Hematoxylin and eosin(HE)staining showed the formation of new granulation tissue and small angiogenesis at the anastomotic site.No colonic epithelium or colorectal glands were found.Masson staining revealed deposition of collagen fibers.Conclusion The rat model of colectomy can be constructed via this protocol,which provides an important tool for exploring the healing mechanism of anastomosis after colectomy.