盐酸丙美卡因对感染性角膜溃疡需氧菌体外培养的影响

目的评估局部眼用盐酸丙美卡因对感染性角膜溃疡需氧菌体外培养的临床效果影响。方法将60例细菌感染性角膜溃疡患者在使用盐酸丙美卡因进行眼部局麻前后分别进行取样,眼部局麻前的样本为对照组,眼部局麻后的样本为试验组。眼部局麻具体处理方法为滴入一滴盐酸丙美卡因入患者眼中大约1 min。比较2组间的初步诊断结果和需氧菌体外培养后的细菌鉴定结果。结果对照组和试验组的初步诊断角膜感染情况为:单一细菌感染率均为88.33%(53例/60例);两种细菌同时感染率分别为8.33%(5例/60例)和10%(6例/60例);多种细菌同时感染率分别为3.33%(2例/60例)和1.67%(1例/60例),差异均无统计学意义(P>0.05)。需氧细菌培养结果显示,对照组和试验组分离最多的均为链球菌(Streptococcus spp),分别占所有分离菌株的55.93%(33株/60株)和52.46%(32株/61株),差异无统计学意义(P>0.05);其次是绿脓杆菌(Pseudomonas aeruginosa),分别占所有分离菌株的13.56%(8株/60株)和14.75%(9株/60株),差异无统计学意义(P>0.05);其他细菌学培养检测结果之间,差异均无统计学意义(P>0.05)。试验组无药物不良反应。结论使用盐酸丙美卡因进行眼部局麻对感染性角膜溃疡的初步诊断结果以及需氧菌体外培养结果无影响。

Effect of promedocaine hydrochloride on aerobic bacteria of infectious corneal ulcer in vitro

Objective To evaluate the clinical effect of local ophthalmic administration of proparacaine hydrochloride on aerobic bacteria of infectious corneal ulcer in vitro.Methods A total of 60 patients with bacterial corneal ulcer were sampled before and after the use of proparacaine hydrochloride for local eye anesthesia.The samples collected before local anesthesia were the control group, and the samples collected after local anesthesia were the treatment group.Local anesthesia is performed by instilling a drop of promedocaine hydrochloride into the eye of the patients for about 1 minute.Then the preliminary diagnosis between the two groups was compared with the identification results of aerobic bacteria cultured in vitro.Results The primary diagnoses of corneal infection in the control and treatment groups were as follows: single bacterial infection rate in both control group and treatment group was 88.33%(53 cases/60 cases);double infection rate were 8.33%(5 cases/60 cases) and 10%(6 cases/60 cases);multiple infection rate were 3.33%(2 cases/60 cases) and 1.67%(1 case/60 cases),all with no statistically significant difference(P > 0.05).The results of aerobic bacterial culture showed that Streptococcus spp was the most isolated bacteria in the control and treated groups,accounting for 55.93%(33 strains/60 strains) and 52.46%(32 strains/61 strains) of all isolated strains,respectively,with no statistically significant difference(P > 0.05);Followed by Pseudomonas aeruginosa,accounted for 13.56% of all isolates(8 strains/60 strains) and 14.75%(9 strains/60 strains),with no statistically significant difference(P > 0.05);There was no statistical difference in the results of other bacteriological cultures(P > 0.05).There were no adverse drug reactions in the treatment group.Conclusion The use of proparacaine hydrochloride for local eye anesthesia has no effect on the preliminary diagnosis of infectious corneal ulcer and the results of in vitro culture of aerobic bacteria.