不同期别矽肺患者肺泡巨噬细胞PRDX4表达差异

目的:探讨不同期别矽肺患者肺泡巨噬细胞（alveolar macrophages，AMs）过氧化物还原酶4（PRDX4）表达的差异。方法:于2017年6月至2018年6月，采用随机抽样方法选取中国煤矿工人北戴河疗养院尘肺科就诊的12名矽肺患者作为研究对象，分为肺内粉尘沉着症组、矽肺壹期组、矽肺贰期组、矽肺叁期组共4组，...

Differential expression of PRDX4 in alveolar macrophages of patients with silicosis

Objective To identify the differential expression of Peroxiredoxin 4(PRDX4)in alveolar macrophages(AMs)from patients with silicosis in different phases using Western blot.Methods From June 2017 to June 2018,Twelve pneumoconiosis patients in the pneumoconiosis Department of Beidaihe Sanitarium were selected by random sampling.A msong them,there were 4 groups,that was lung with dust,silicosis with grade one,silicosis with grade two,silicosis with grade three.There were 3 persons in each group,a total of 12.AMs was obtained by filtration and centrifugation.The intracellular protein was extracted and PRDX4 was detected by using Western blot method.Results It results showed that PRDX4 was expressed in AMs in 4 groups;with the increase of fibrosis,the average relative expression of PRDX4 in AMs was 0.258±0.026,0.214±0.012,0.180±0.004,0.165±0.008.The highest expression level was in the lung with dust group,and the lowest was in the silicosis with grade three group.The difference of the expression level of PRDX4 protein in AMs between groups was statistically significant(P<0.05).Conclusion This experiment verified that PRDX4 expressed differentially in AMs in patients with silicosis.With the development of silicosis,PRDX4 expression in AMs reduced significantly.