根皮苷对肥胖小鼠糖脂代谢的调控作用

目的探究根皮苷对肥胖小鼠糖脂代谢的调控作用及对肝脏组织哺乳动物的雷帕霉素靶蛋白(mTOR)蛋白表达的影响。方法 60只小鼠随机分为对照组(n=20)、模型组(n=20)及实验组(n=20),对照组正常饲养,模型组及实验组小鼠则连续饲喂8周高脂高糖饲料构建肥胖小鼠模型。建模后实验组灌服80 mg·kg-1根皮苷,模型组及对照组灌服等量生理盐水,每天1次,连续干预14周。血糖仪检测小鼠空腹血糖(FPG)水平;全自动生化分析仪检测小鼠血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固(LDL-C)及高密度脂蛋白胆固醇(HDL-C)水平;蛋白质印迹法检测肝脏组织mTOR及磷酸化mTOR (p-mTOR)蛋白表达。结果对照组,模型组及实验组血清FPG水平分别为(5.22±1.24),(9.69±1.46),(6.43±1.33)mmol·L-1;TC水平分别为(1.96±0.75),(4.81±0.99),(2.40±0.81)mmol·L-1;TG水平分别为(1.21±0.46),(3.73±0.57),(1.34±0.51)mmol·L-1;LDL-C水平分别为(1.86±0.62),(4.55±0.80),(2.18±0.74)mmol·L-1;HDL-C水平分别为(2.79±0.91),(1.11±0.59),(2.48±0.65)mmol·L-1;肝组织中mTOR蛋白相对表达量分别为0.49±0.04,0.93±0.06,0.67±0.05;p-mTOR蛋白相对表达量分别为0.53±0.03,0.68±0.04,0.42±0.03。模型组小鼠血清FPG、TC、TG、LDL-C水平及肝组织mTOR、p-mTOR蛋白相对表达量均较对照组升高,而实验组较模型组降低;血清HDL-C水平较对照组降低,而实验组较模型组升高(均P<0.05)。结论根皮苷可有效调节肥胖小鼠糖脂代谢,其作用机制可能与有效抑制肝脏组织mTOR蛋白表达及其磷酸化相关。

Regulation effect of phlorizin on the glucose and lipid metabolism of obese mice

Objective To explore the regulation effect of phlorizin on the glucose and lipid metabolism of obese mice and influence on liver tissue mammalian target of rapamycin(mTOR) protein expression.Methods 60 healthy, male C57 BL/6 J mice were divided randomly into control group, model group and test group, 20 cases were for each group, mice in control group treated with normal breeding, mice in model group and test group were built obese mice model by treating with feeding high fat and sugar feed for continuous 8 weeks, after the success of the modeling, the mice in test group received gastrointestinal administration with 80 mg·kg-1 phlorizin, and mice in model group and test group gastrointestinally administrated with the same amount of saline, 1 time per day, intervention for continuous 14 weeks.The fasting blood glucose(FPG) level of mice were detected by glucose meter, the serum triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) levels were detected by automatic biochemical analyzer;the m TOR and phosphorylation of m TOR(p-m TOR) proteins expression were detected by Western blot.Results The serum FPG levels in control group,model group and test group were(5.22 ± 1.24),(9.69 ± 1.46),(6.43 ± 1.33)mmol·L-1;TC levels were(1.96 ± 0.75),(4.81 ± 0.99),(2.40 ± 0.81) mmol·L-1;TG levels were(1.21 ± 0.46),(3.73 ± 0.57),(1.34 ± 0.51) mmol·L-1;LDL-C levels were(1.86 ± 0.62,)(4.55 ± 0.80),(2.18 ± 0.74) mmol·L-1;HDL-C levels were(2.79 ± 0.91),(1.11 ± 0.59),(2.48 ± 0.65) mmol·L-1;the relative expression of m TOR protein in liver tissue were 0.49 ± 0.04,0.93 ± 0.06,0.67 ± 0.05;the relative expression of p-m TOR protein in liver tissue were 0.53 ± 0.03,0.68 ± 0.04,0.42 ± 0.03.The serum FPG,TC,TG,LDL-C levels and the relative expression of m TOR,p-m TOR in live tissue of mice increased compared with control group,while test group was lower than model group;the serum HDL-C levels decreased compared with control group,while test group was higher than model group(all P < 0.05).Conclusion Phlorizin can adjust the glucose and lipid metabolism of obese mice,the action mechanism may be related to inhibition of liver tissue m TOR protein expression and phosphorylation.