右美托咪定对小鼠树突状细胞免疫功能的影响

目的研究右美托咪定对小鼠树突状细胞功能的影响及作用机制。方法分离获取小鼠骨髓原性树突状细胞鉴定成功后,体外分组培养,以正常培养基作为空白组,实验组为含有10μmol·L-1右美托咪定的正常培养基,对照1组和对照2组分别为含有10μmol·L-1酚苄明及同时含有10μmol·L-1右美托咪定和酚苄明的正常培养基,分别于96孔板培养24 h、Transwell 24孔板培养4 h和6孔板培养24 h。测定实验药物对小鼠树突状细胞活力、细胞迁移数量及肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)的影响情况。结果空白组、实验组、对照1组和对照2组的树突状细胞活力分别为(99.96±0.19)%,(62.61±0.08)%,(98.74±0.45)%和(79.25±0.23)%,树突状细胞迁移细胞数量分别为(3684.68±38.36),(1836.37±21.38),(3659.90±27.92)和(2670.06±27.61)个,树突状细胞培养上清液的TNF-α浓度分别为(67.26±1.51),(353.81±6.29),(67.92±1.45)和(169.63±2.89)pg·mL-1,IL-6浓度分别为(29.64±1.20),(511.29±12.97),(29.38±1.88)和(230.17±2.19)pg·mL-1,IL-1β浓度分别为(46.51±2.43),(436.97±5.86),(46.19±1.29)和(147.30±3.57)pg·mL-1,实验组与其他组比较,差异均有统计学意义(均P<0.05)。结论右美托咪定能够影响小鼠树突状细胞的活力、迁移能力以及促炎性细胞因子的分泌,其作用机制跟α肾上腺素受体的激活有关。

Effect of dexmedetomidine on immune function of mouse dendritic cells

Objective The effect of dexmedetomidine on mouse dendritic cell function and its mechanism.Methods After the isolation and identification of mouse bone marrow-derived dendritic cells were successfully identified, they were cultured in groups in vitro, and normal medium was used as blank group.The experimental group contained 10 μmol·L-1 dexmedetomidine, control 1 and control 2 groups contained 10 μmol·L-1 phenoxybenzamine and also contained 10 μmol·L-1 dexmedetomidine and phenoxybenzamine, respectively, were cultured in 96-well plates for 24 h, Transwell 24-well plates for 4 h and 6-well plates for 24 h.The effects of experimental drugs on the viability of mouse dendritic cells, the number of cell migration, and the pro-inflammatory cytokines tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-1β(IL-1β) were determined.Results The viability of dendritic cells in blank group, experimental group, control 1 group and control 2 group were(99.96±0.19)%,(62.61 ± 0.08) %,(98.74 ± 0.45) % and(79.25 ± 0.23) %,the number of migrating dendritic cells were(3684.68 ± 38.36),(1836.37 ± 21.38),(3659.90 ± 27.92) and(2670.06 ± 27.61) cells,TNF-α in the culture supernatant of dendritic cells were(67.26 ± 1.51),(353.81 ± 6.29),(67.92 ± 1.45) and(169.63 ± 2.89) pg·mL-1,IL-6 concentration were(29.64 ± 1.20),(511.29 ± 12.97),(29.38 ± 1.88) and(230.17 ± 2.19) pg·mL-1,IL-1β concentration were(46.51 ± 2.43),(436.97 ± 5.86),(46.19 ± 1.29) and(147.30 ± 3.57) pg · mL-1.The differences between the experimental group and other groups were statistically significant(all P < 0.05).Conclusion Dexmedetomidine can affect the viability,migration ability and secretion of pro-inflammatory cytokines of mouse dendritic cells,and its mechanism of action is related to the activation of α-adrenergic receptors.