粪便多配体聚糖2基因甲基化在结直肠恶性肿瘤进展的相关性

目的:探讨粪便多配体聚糖2(SDC2)基因甲基化在结直肠恶性肿瘤(CRC)进展中的相关性。方法:收集2018年4月至2019年7月上海交通大学医学院附属松江医院经结肠镜检查(和/或)病理诊断的133例受试者，其中44例CRC、61例进展期腺瘤(AA)和28例对照者(CRC和AA均为阴性者)为研究对象。每例收集两份粪便标...

Correlation between fecal syndecan-2 gene methylation and colorectal carcinoma progression

Objective To investigate the correlation between fecal syndecan-2(SDC2)gene methylation and colorectal carcinoma(CRC)progression.Methods A total of 133 subjects diagnosed by colonoscopy(and/or)pathology in Songjiang District Central Hospital from April 2018 to July 2019 were collected and divided into three groups:CRC group(n=44),advanced adenoma group(AA group)(n=61)and control group(CRC-and AA-)(n=28).With the prospective double-blind paired method,two fecal samples were collected from each case,and tested by real time quantitative methylation specific polymerase chain reaction(PCR)(qMSP)(SDC2 methylation)and fecal immunochemical test(FIT)respectively,and the sensitivity and specificity of the two methods were compared.In combination with clinical postoperative pathological specimens and staging,Fisher exact probability test was used to analyze the counting data,Rank sum test of Kruskal-wallis method(among the three groups)and Mann-Whitney method(between the two groups)were used to analyze the quantitative data,and Spearman correlation was analyzed between the Ct value of SDC2 methylation in CRC group and tumor diameter,staging,depth of infiltration and lymph node metastasis.Results The sensitivity of SDC2 methylation in CRC group and AA group was both significantly higher than that of FIT(0.909 vs.0.614,P<0.05 and 0.525 vs.0.164,P<0.01).The Ct values of SDC2 methylation in CRC group,AA group and control group were M(P25-P75):35.02(31.32-37.87),38.90(37.26-51.00)and 51.00(40.22-51.00).The Ct values of SDC2 methylation in CRC group were significantly lower than those in AA group,while those in AA group were significantly lower than those in control group(Z=-5.213,-4.048,P<0.01).Univariate correlation analysis showed that the Ct value of SDC2 methylation in CRC group was negatively correlated with tumor diameter,staging,invasion depth and lymph node metastasis(r=-0.422,-0.437,-0.371,-0.417,P<0.05),and positively correlated with age(r=0.308,P<0.05).Conclusion The sensitivity of fecal SDC2 methylation in CRC and AA examination is significantly higher than that of FIT,and the degree of SDC2 methylation is positively correlated with the degree of CRC tumor progression.