重楼皂苷Ⅰ抗肝癌细胞作用的初步研究

目的:本文旨在研究重楼皂苷Ⅰ对肝癌细胞株Huh7和HepG2的抑制作用并探讨其是否具有抑制肝癌干细胞的功效.方法:不同剂量的重楼皂苷Ⅰ处理肝癌细胞后,MTT方法检测细胞存活情况;流式细胞术检测细胞凋亡情况;Transwell实验检测细胞迁移能力;磁珠分选出CD133+-Huh7和CD133--HepG2细胞后,成球实验...

Preliminary study for effect of Polyphyllin I on hepatocellular carcinoma cells

Objective:To study the inhibitory effect of PolyphyllinⅠon Huh7 and HepG2 hepatoma cell lines and to explore whether it has the inhibitory effect to hepatocellular carcinoma stem cells.Methods:After hepatoma cells were treated with different doses of PolyphyllinⅠ,MTT assay was used to detect cell survival,flow cytometry was used to detect cell apoptosis,Transwell was used to detect cell migration.CD133+-Huh7 and CD133+-HepG2 cells were separated by magnetic beads.The effect of Polyphyllin I on the sphere-forming ability of CD133+and CD133-hepatoma cells was detected by spheroidization test.Results:PolyphyllinⅠcould inhibit the proliferation and clone formation of Huh7 and HepG2 cells,and inhibit the migration of hepatocellular carcinoma cells.Moreover,PolyphyllinⅠcan induce dose-dependent apoptosis of hepatocellular carcinoma cells.The results of magnetic separation and pelletization in vitro showed that PolyphyllinⅠmade the cells smaller and the cell morphology shrunk,which had no difference in effect on CD133+-Huh7 and CD133+-HepG2,made cells were smaller,cell morphology shrivel.Conclusion:PolyphyllinⅠcan inhibit the proliferation,migration and induce apoptosis of Huh7 and HepG2 hepatocellular carcinoma cells,but it has no obvious the ability to kill hepatocellular carcinoma stem cells.