| 急性弛缓性麻痹病例监测中影响病毒分离敏感性的因素 |
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| 引用本文： | 柯昌文,林协勤,郑焕英,邵晓萍,吴承民. 急性弛缓性麻痹病例监测中影响病毒分离敏感性的因素[J]. 中国疫苗和免疫, 1997, 0(2) |
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| 作者姓名： | 柯昌文 林协勤 郑焕英 邵晓萍 吴承民 |
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| 作者单位： | 广东省卫生防疫站 |
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| 摘 要： | 广东省１９９２－１９９５年急性弛缓性麻痹（ＡＦＰ）病例粪便标本病毒学监侧结果表明，ＡＦＰ病例出现麻痹后，间隔２４－４８小时及时采集双份合格粪便标本，可显著提高病毒分离的阳性率。同时使用ＲＤ细胞系大大提高了非脊髓灰质炎（脊灰）肠道病毒（ＮＰＥＶ）的检出率。为证实消灭脊灰，在今后的ＡＶＰ病例监测中，必须进一步提高监测质量，尽早采集双份合格粪便标本，做好实验室病毒检测工作．
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| 关 键 词： | 急性驰缓性麻痹，脊髓灰质炎病毒，非脊髓灰质炎肠道病毒，监测 |
FACTORS INFLUENCING THE SENSITIVITY OF VIRAL ISOLATION DURING ACUTE FLACCIDPARALYSIS SURVEILLANCE |
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| Ke Changwen Lin Xieqin Zheng Huanying Shao Xiaoping Wu Chengmin. FACTORS INFLUENCING THE SENSITIVITY OF VIRAL ISOLATION DURING ACUTE FLACCIDPARALYSIS SURVEILLANCE[J]. Chinese Journal of Vaccines and Immunization, 1997, 0(2) |
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| Authors: | Ke Changwen Lin Xieqin Zheng Huanying Shao Xiaoping Wu Chengmin |
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| Abstract: | This paper analyzed the virological surveillance results of acute flaccid paralysis(AFP). Stool specimens were collected from 1992 to 1995 in Guangdong Province. The results showed that the sensitivity of viral isolation could be improved significantly if two ade- quate stool samples were collected at least 24 - 48 hours apart, within 14 days of the onset of paralysis . The study also confirmed that RD cell line could increase the positive isola- tion rate of non - polio enterovirus (NPEV). Therefore the mouse cell line expressing hu- man gene for poliovirus receptor should be used in routine work in the polio laboratory. |
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| Keywords: | Acute flaccid paralysis surveillance Poliovirus Sensitivity Non - polio enterovirus |
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