运动、锌铜营养与自由基代谢——Ⅵ.不同量锌补充对缺锌运动小鼠体内自由基生成与清除的影响

离乳Balb/c小鼠64只,按性别体重随机分成继续缺锌、缺锌后补锌5ppm、缺锌后补锌50ppm及缺锌后补锌500ppm四大组,每大组又分成运动和不运动两组。开始时所有小鼠均进食缺锌饲料。6周后继续缺锌组小鼠继续进食缺锌饲料,其余各组则分别进食补锌5、50和500ppm的补锌饲料。又3周后,所有小鼠均处死并取血、肝进行锌、铜、丙二醛(MDA)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px) 的测定。结果提示:补锌50ppm对运动及不运动缺锌小鼠各项指标的恢复均有良好作用;补锌5ppm对运动小鼠非最佳剂量。补锌500ppm无论对运动小鼠还是不运动小鼠均有害。

EFFECT OF ZINC REPLETION ON THE FREE RADICAL GENERATION AND THE FREE RADICAL DEFENSE SYSTEM IN EXERCISED MICE

64 weanling mice were randomly divided into four groups according to their sex and weight: group A,B, C and D. Half of each group received an exercise training program consisting of swimming for 60 minutesper day in deionized water. All the mice were fed a zinc-deficient diet containing 1.6 ppm zinc for 6 weeksat first. Then the mice in group A were fed the zinc-deficient diet continuously and the mice in group B, Cand D were supplemented with 5, 50 and 500 ppm zinc in the zinc-deficient diet respectively for 3 weeks.Zinc, copper, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)were measured after the mice were killed by decapitation. The result showed that 50 ppm zinc supplemented in diet was a adequate to correct the zinc deficientstatus in both sedentary and exercised mice, while 5 ppm zinc had a good effect only on sedentary mice. 500ppm zinc even had a harmful effect on both sedentary and exercised mice.