Brown spider (Loxosceles intermedia) venom triggers endothelial cells death by anoikis |
| |
Authors: | Jenifer NowatzkiReginaldo Vieira Sene,Katia Sabrina Paludo,Luiz Eduardo RizzoFernando Souza-Fonseca-Guimarã es,Silvio Sanches VeigaHelena Bonciani Nader,Cé lia Regina C. FrancoEdvaldo S. Trindade |
| |
Affiliation: | a Department of Cell Biology, Federal University of Paraná, 81351-980, Curitiba, PR, Brazil b Department of Basic Pathology, Federal University of Paraná, 81531-980, Curitiba, PR, Brazil c Department of Biochemistry, Federal University of São Paulo, 04044-020 São Paulo, SP, Brazil d Department of Cell Biology, Molecular and Genetics, Estadual University of Paraná, 84030-900, PR, Brazil |
| |
Abstract: | Brown spider (Loxosceles sp.) venom affects the endothelium of vessels and triggers disruptive activity in the subendothelial matrix. The vascular disorders observed after venom exposure include leukocyte and platelet activation, disseminated intravascular coagulation, an increase in vessel permeability and hemorrhage into the dermis. In this study, we report additional evidence regarding the mechanism of endothelial cell cytotoxicity induced by Loxosceles intermedia venom. Exposure to venom led to endothelial cell detachment in a time-dependent manner. Loss of cell anchorage and cell-cell adhesion following venom exposure was accompanied by changes in the distribution of the α5β1 integrin and VE-cadherin. An ultrastructural analysis of cells treated with venom revealed morphological alterations characteristic of apoptosis. Moreover, after venom exposure, the ratio between Bax and Bcl-2 proteins was disturbed in favor of Bax. In addition, late apoptosis was only observed in cells detached by the action of venom. Accordingly, there was no increase in apoptosis when cells were exposed to L. intermedia venom in suspension, suggesting that the loss of cell anchorage provides the signal to initiate apoptosis. Thus, L. intermedia venom likely triggers endothelial cell death indirectly through an apoptotic mechanism known as anoikis. |
| |
Keywords: | Loxosceles intermedia Venom Anoikis Apoptosis Endothelial cells |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|