Brain-derived neurotrophic factors increase the proliferation and differentiation of endogenous neural stem cells in mouse models of cerebral infarction
1. Department of Neurology, Tianjin First Central Hospital, Tianjin 300192, China;2. Division of Microbiology, Department of Laboratory, Tianjin First Central Hospital, Tianjin 300192, China;3. Howard Florey Institute, Medical College, the University of Melbourne, Australia
Abstract:
BackgroundIt has been confirmed that brain-derived neurotrophic factor (BDNF) can promote the proliferation of neural stem cells (NSCs) and protect neuron-like cells in vitro. However, its effect on endogenous NSCs in vivo is still unclear.ObjectiveTo evaluate whether BDNF can induce the endogenous NSCs to proliferate and differentiate into the neurons in the mice model of cerebral infarction.DesignA synchronal controlled observation.SettingsDepartment of Neurology, Microbiology Division of the Department of Laboratory, Tianjin First Central Hospital; Howard Florey Institute, Medical College, the University of Melbourne.MaterialsTwenty-four pure breed C57BL/6J mice at the age of 10 weeks old (12 males and 12 females) were divided into saline control group and BDNF-treated group, 6 males and 6 females in each group.MethodsThe experiments were performed at the University of Melbourne from July 2004 to February 2005.
