| 蒙药耧斗菜总生物碱的测定及提取工艺研究 |
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| 引用本文： | 张园园,敖恩宝力格,白雪莲.蒙药耧斗菜总生物碱的测定及提取工艺研究[J].北方药学,2014,0(11):21+137-21. |
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| 作者姓名： | 张园园 敖恩宝力格 白雪莲 |
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| 作者单位： | 内蒙古师范大学生命科学与技术学院 呼和浩特 010022 |
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| 基金项目： | 内蒙古自治区自然科学基金项目2014MS0340。 |
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| 摘 要： | 目的：研究蒙药耧斗菜中总生物碱的含量及提取工艺条件。方法：采用有机溶剂提取法用不同乙醇浓度、不同提取条件对耧斗菜总生物碱进行提取,含量测定采用分光光度法。结果：耧斗菜总生物碱含量较高,1g耧斗菜干粉中提取到的总生物碱含量为0.155mg,其总生物碱提取率为0.016%。提取最佳乙醇浓度为80%,固液比为1∶25,提取温度为60℃,浸提时间为60min。结论：蒙药耧斗菜在总生物碱提取开发利用方面有较好的应用前景。
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| 关 键 词： | 耧斗菜 生物碱 提取条件 |
Study on Extraction conditions and Content determination in total alkaloids in Aquilegia viridiflora |
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| Zhang Yuanyuan,Aoen Bolige,Bai Xuelian.Study on Extraction conditions and Content determination in total alkaloids in Aquilegia viridiflora[J].BEIFANG YAOXUE,2014,0(11):21+137-21. |
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| Authors: | Zhang Yuanyuan Aoen Bolige Bai Xuelian |
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| Institution: | Zhang Yuanyuan;Aoen Bolige;Bai Xuelian;College of Life Science and Technology;Inner Mongolia Normal University; |
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| Abstract: | To Study the optimum extraction conditions and content determination of total alkaloids in Aquilegia viridiflora. Extract the total alkaloids by organic solvent extraction method, with different ethanol concentration and different extraction conditions .Content determination by spectrophotometry . The content of total alkaloids was higher, lg Aquilegia have 0.155mg alkaloid, the extraction rate of the total alkaloids was 0.016%. the optimum condition was as follows： ethanol concentration 80%, solid-to-liquid ratio of 1：25, 60℃ for 60 minutes. |
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| Keywords: | Aquilegia viridiflora Alkaloid Extraction condition |
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