大鼠全脑缺血再灌注后海马区细胞凋亡及bcl-2和Bax的检测

目的：证实缺血性脑损伤主要是以细胞凋亡的方式发生。方法；用ＴＵＮＥＬ法标记检测损伤的神经细胞，用原位杂交组织化学法检测ｂｃｌ－２ ｍＲＮＡ，用免疫组织化学法检测Ｂａｘ蛋白。结果：手术组检测到ＴＵＮＥＬ标记的阳性神经细胞主要位于海马ＣＡ１区，渐进性增多，第３天达到高峰，可检测到凋亡小体和正在形成凋亡小体的神经细胞。

Cell apoptosis in hippocampus after cerebral ischemia, reperfusion and detection of bcl-2 and Bax

Objective:To study the mode of cell death ocurring in the delayed neuronal death after rat transient global ischemia. Methods:TUNEL method was used to morphologically detect the injured nerve cells, immunohistochemistry staining to detect Bax and in situ hybridization to detect bcl-2 mRNA. Results:The results were as follows: TUNEL-positive neurons were stained in hippocampuses of operated rats ,which was mostly located in CA1 zone, increased as time passed, reached max after day 3. Apoptotic bodies and the neurons forming the apoptotic bodies were also found. Bcl-2 mRNA positive signals were widespread in CA3 pyramidal neurons. On the contrary,Bax-positive neurons was mostly located in the CA1 subfield.These 2 signals occurred on reperfusion 8 h,reached max on 24 h and markedly reduced on 72 h.Conclusion:The findings strongly suggest :(1)Apoptosis may contribute to the delayed neuronal death .(2) Selected expression of different members of bcl-2 gene family may play an important role in determining the relative sensitivity of neuronal subpopulations to ischemia.