虎丹颗粒对高脂血症小鼠血脂质代谢及氧自由基的影响

目的探讨虎丹颗粒对高脂血症小鼠血脂质代谢及氧自由基的影响.方法78只小鼠随机分为正常对照组、高脂血症组、虎丹颗粒高、中、低剂量组和辛伐他汀对照组.除正常对照组外,其他组小鼠灌服脂肪乳剂建立高脂血症模型,虎丹颗粒按12、6、3 g/kg剂量给小鼠连续灌胃4周后,测定血清总胆固醇(TC)、甘油三酯(TG)、高密度胆蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性及脂质过氧化物(LPO).结果与高脂血症组相比,虎丹颗粒能明显降低TC、TG和LDL-C的浓度(P＜0.05),能明显升高血SOD和GSH-Px活性(P＜0.05),降低血清LPO的浓度(P＜0.05).与辛伐他汀组相比,虎丹颗粒在降脂方面稍差(P＜0.05),但在清除氧自由基活性方面要优于辛伐他汀(P＜0.05).结论虎丹颗粒具有能调节小鼠高脂血症脂质代谢和增强抗脂质过氧化的作用.

Effects of HuDan Granules on Lipid Metabolism and Antioxidant Activity of Mice with Hyperlipidemia

OBJECTIVE: To study the effects of HuDan Granules on lipid metabolism and antioxidant activity of mice with hyperlipidemia. METHODS: Seventy-eight mice were divided into six groups randomly: the normal control group, hyperlipemia group, HuDan Granules high, middle, low dose group and the Simvastatin group. Besides the normal control group, all the mice in the other groups were fed with lipid emulsion for 4 weeks. The Chinese medicine groups were treated with HuDan Granules at the dose of 12,6,3 g/kg, the normal control group and hyperlipemia group were treated with normal sodium, the Simvastatin group were treated with Simvastatin. After 4 weeks, the TC, TG, HDL-C, LDL-C, SOD, GSH-Px and LPO in serum were measured. RESULTS: HuDan Granules groups could significantly decrease the serum TC, TG, HDL-C in the experimental hyperlipidemia mice, and markedly increase the level of serum HDL-C. Mean level of serum LPO in the experimental groups treated by HuDan Granules at different dosages were much lower than that in hyperlipidemia group (P<0.05), but higher than the Simvastatin group (P<0.05). And the SOD and GSH-Px activities of serum in the group of HuDan Granules were much higher than those in hyperlipidemia group and the Simvastatin group (P<0.05). CONCLUSION: HuDan Granules can regulate lipid metabolism, enhance the antioxidation and reduce the lipid peroxidation in mice with hyperlipidemia.