生长抑素2型受体mRNA在人胃上皮永生细胞及肿瘤细胞株中的表达

目的 了解多种癌细胞上是否较广泛表达生长抑素 2型受体，同时了解 p53突变与否对生长抑素 2型受体的表达有无影响。方法 用 RT-PCR和克隆的人生长抑素 2型受体 cDNA为探针检测人胃上皮永生细胞、人胃癌细胞、胰腺癌细胞、结肠癌细胞、膀胱癌细胞共 10株细胞上该受体 mRNA的表达。结果 发现除 1株胰腺癌细胞外，其余 9个细胞株均表达生长抑素 2型受体。一株胃癌细胞分别转染了野生型和突变型的 p53基因，也同样表达生长抑素 2型受体。 结论 提示生长抑素 2型受体在癌细胞的表达有较大的普遍性，在胃癌细胞中生长抑素 2型受体的表达与 p53是否突变可能无关。

Somatostatin Receptor Subtype 2 mRNA Expression in Human Gastric Immortalized Cell Line and Tumor Cell Lines

Inhibition of somatostatin on cell proliferation via somatostatin receptor subtype 2 (SS-R2), suggested this receptor subtype may be involved in tumor growth. Present study is to demonstrate whether SS-R2 was expressed in tumor cell lines extensively as well as in human gastric epithelia immortalized cell line. Meanwhile, the effect of p53 mutation on SS-R2 expression in gastric cancer cell line was also elucidated. Methods Full-length human somatostatin receptor subtype 2 cDNA was cloned by RT-PCR. Using cDNA as the probe and RT-PCR, expression of mRNA for SS-R2 in 9 cancer cell lines and human gastric immortalized cell line was detected. Results Data revealed that most cancer cell lines but a pancreatic cancer cell line expressed SS-R2 mRNA. Conclusion Regardless whether p53 mutation or not in human gastric cancer cell lines, SS-R2 mRNA was transcribed.