河豚鱼干中毒的动物实验研究及在法医鉴定中的应用

目的:研究河豚鱼干中的毒性成分对小白鼠肝、肾等脏器的损害。方法:应用乙酸浸泡法提取涉案河豚鱼干中的含毒浸出液,在1h、2h、3h、4h和6h共5个不同时间段,对小白鼠采用灌胃和腹腔注射方法,制造实验中毒模型。结果:实验3h、4h组损伤最明显,死亡率达100%;河豚鱼毒素(Tetrodotoxin,TTX)导致实验小白鼠的肝、肾组织细胞中毒坏死,所有实验动物均出现呼吸急促、步态不稳和口尾紫绀等TTX中毒症状。结论:实验证实检案确系河豚鱼干中毒。

Application of study toxic by tetrodotoxin in the forensic identify

Objective:To study the pathology change and toxic mechanism of tetrodotoxin.Methods:To concentrations of tetrodotoxin in biological tissue were analyzed by soak with acetic acid,five groups of rats were fed by perfuse from stomach or injected from abdominal and they were killed at 1 hour,2 hours,3 hours,4 hours and 6 hours,respectively.Results:The injury in 3 hours and 4 hours rat groups were the most obviously and the death rate was 100%.The tetrodotoxin disrupted the tissues of livers and kidneys.All the rats showed the intoxic symptom of tachypnea,instability of gait and oral lips cyanosis.Conclusion:To clean the toxicant the case of intoxication by tetrodotoxin should be emeticed,gastric lavage and cathartic as soom as possible.