| HIV-1整合酶单链抗体在大肠杆菌中的表达、纯化与鉴定 |
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| 引用本文： | 张健慧,宋晓国,孟莉. HIV-1整合酶单链抗体在大肠杆菌中的表达、纯化与鉴定[J]. 细胞与分子免疫学杂志, 1999, 0(1): 7 |
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| 作者姓名： | 张健慧 宋晓国 孟莉 |
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| 作者单位： | ：张健慧 宋晓国 孟莉 冯玉英 韩保光 凌世淦 马贤凯(军事医学科学院基础医学研究所，北京100850） |
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| 摘 要： | 将具有HIV－1整合酶结合活性的单链抗体重组噬菌体感染HB2151大肠杆菌，经IPTG诱导．在周质腔萃取物及培养基上清中，均检测到有较特异与整合酶结合的可溶性单链抗体的表达。利用HiTrapAnti－Etags对单链抗体进行了亲和层析纯化。Western-blot结果初步表明，单链抗体不仅可与整合酶的单体反应，而且也可与其二聚体结合。提示：研制的单链抗体可用于对HIV－1整合酶活性影响的研究。
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| 关 键 词： | 人类免疫缺陷病毒 整合酶 单链抗体 |
Expression, purificationand identification of single chain fragments variable(ScFv) antibody against HIV |
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| Zhang Jianhui, Song Xiaoguo, Meng Li, Feng Yuying, Han Baoguang, Ling Shigan, Ma Xiankai. Expression, purificationand identification of single chain fragments variable(ScFv) antibody against HIV[J]. Chinese journal of cellular and molecular immunology, 1999, 0(1): 7 |
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| Authors: | Zhang Jianhui Song Xiaoguo Meng Li Feng Yuying Han Baoguang Ling Shigan Ma Xiankai |
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| Abstract: | E. ed HBZ151 was infected with weombinant HIV-1 integrase-binding ScFv-phages. After induced by IPTG, expmsion of Soluble ScFv charactehaed by spec binding to iN pekin was detected in the hacteda periplasm. and supemawt of cultare inwhwn. Using HiTraP Anti-E tag column, the ScFv was Pnded by amity cluolnatopohy. The prelims~ ~its of Western-blot showed that the ScFv could bind tO hath oligomer and dimer of aam Protein. 11 was iwhcated that the ScFv could be used for stUdy its possible the in inhibiting IN activities. |
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| Keywords: | HIV integrase ScFv |
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