| 一种快速、简便、敏感、特异的白细胞介素2、3、6生物学检测法：MTS／PMS法的建立 |
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| 引用本文： | 章卫平,曹雪涛.一种快速、简便、敏感、特异的白细胞介素2、3、6生物学检测法：MTS／PMS法的建立[J].中国免疫学杂志,1994(3). |
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| 作者姓名： | 章卫平 曹雪涛 |
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| 作者单位： | 第二军医大学免疫学教研室 |
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| 基金项目： | 上海市科技启明星计划资助 |
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| 摘 要： | ＭＴＴ的一种新型类似物ＭＴＳ在ＰＭＳ存在情况下可被活细胞还原，形成水溶性甲产物。本实验表明，将ＭＴＳ／ＰＭＳ取代ＭＴＴ用于ＩＬ－２、３、６生物学测定法取得理想结果，ＭＴＳ／ＰＭ５形成的比色产物与细胞数量、ＭＴＳ／ＰＭＳ与细胞孵育时间呈正相关。ＭＴＳ／ＰＭＳ生物学测定法省略了加有机溶剂溶解甲的步骤，ＭＴＳ／ＰＭ５贮存稳定、显色快速，故该法具有快速、简便敏感、特异的优点，适用于细胞因子的生物学测定。
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| 关 键 词： | 细胞因子生物学测定法，比色测定法，白细胞介素2，白细胞介素3，白细胞介素6 |
ESTABLISHMENT OF A RAPID,SIMPLE,SENSITIVE AND SPE-CIFIC BIOASSAY FOR
INTEKLEUKIN-2,3,6:MTS/PMS BIOAS-SAY |
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| Zhang Weiping,Cao
Xuetao.ESTABLISHMENT OF A RAPID,SIMPLE,SENSITIVE AND SPE-CIFIC BIOASSAY FOR
INTEKLEUKIN-2,3,6:MTS/PMS BIOAS-SAY[J].Chinese Journal of Immunology,1994(3). |
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| Authors: | Zhang Weiping Cao
Xuetao |
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| Abstract: | new analog of
MTT,MTS(3-(4,5-dimethylthiazol-2-yl)-5-( 3-car
boxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium,inner salt)could
be reduced by living cells to produce a wa-ter-soluble formazan
product in the presence of phenazine methasulfate(PMS).MTS/PMS
wastised as a substitute for MTT in the bioassay for interleukin-2,3,
6 using dependent cell lines andgood results had been achieved.Our
experiment showed that the ammount of colored productformed by
MTS/PMS was proportional to the number of living cells and the time
of incubationof the cells with MTS/PMS.The advantages of MTS/PMS
bioassay include the-elimination ofsteps for organic solvent
solubilization , the storage stability of MTS/PMS reagents and
fastercolor development,etc.These results demonstrated that MTS/PMS
bioassay was rapid,Sim-ple, sensitive,specific,and was very
applicable for cytokine assay. |
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| Keywords: | Cytokine bioassay Colorimetric assav IL-2 IL-3 IL-6 |
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