E838对辐射所致小鼠淋巴细胞DNA双链断裂的防护作用

目的：探讨E838对辐射导致小鼠淋巴细胞DNA双链断裂的防护作用。方法：小鼠照射前给予E838，观察其存活率和动物平均存活时间；用中性单细胞凝胶电泳技术，检测1 Gyγ射线照射后小鼠淋巴细胞DNA双链断裂，并与给予同类药物小鼠进行比较，用CASP软件分析彗星图像，SPSS12．0进行数据的统计学分析。结果：E838预防用药能够明显提高损伤动物的30天存活率，E838低、中、高剂量组存活率分别比对照组提高55%、85%、75%，平均生存天数与对照组比较，差异有统计学意义（P<0.001），保护指数分别为1.99、2.56、2.46。E838与炔雌醇比较，中、高剂量组的动物平均生存天数明显延长，差异有统计学意义（P<0.001）。E838能明显减轻淋巴细胞DNA双链断裂损伤，E838低、中、高组，TDNA百分比（11.68、6.24、9.05）、TL（18.53、10.09、16.02）、TM（2.13、0.64、1.21） 和OTM（2.52、0.99、1.59 ）残余损伤逐渐减少，与对照组比较，差异有统计学意义(P<0.001)。E838与炔雌醇、尼尔雌醇比较差异有统计学意义(P<0.05)。结论：E838对辐射损伤具有明显的保护作用。

Protective Effects of E838 on Mouse Lymphocyte Double-strand DNA Broken Induced by Radiation

Objective：To investigate the effects of E838 on mouse survival after lethal dose irradiation and double-strand DNA broken in mouse lymphocyte induced by radiation.Methods：E838 was given intraperitoneal injection before radiation.The survival time and mortality of mouse were observed.Double-strand DNA broken after 1 Gy γ radiation was detected by single cell gel electrophoresis assay technology and the results was compared with positive drug.The comet image was analyzed by using CASP software and SPSS 12.0 was used for statistical analysis.Results：The 30d survival time of E838 groups were significantly increased compared with that of control group.Compared with the control group,survival rate in group of E838 with high dose,middle dose and low dose increases by 55 percent,85 percent,75 percent respectively,and it has a significant difference in the survival time(P<0.001).The protective index is 1.99,2.56,2.46 respectively.Compared with Ethinylestradiol, mouse in group of E838 with middle dose and high dose has longer survival time (P<0.001). The double-strand DNA broken in lymphocytes was obviously decreased when mice were preventively given E838 before irradiation.The group of E838 with high dose，middle dose and low dose， the percentage of TDNA（11.68,6.24,9.05）,TL （18.53,10.09,16.02）,TM（2.13,0.64,1.21） and OTM（2.52,0.99,1.59） have a decrease in residual damage(P value<0.001).It has a significant difference among E838,Ethinylestradiol and Nylestriol.Conclusion：E838 has remarkable protective effects on DNA damage after γ-irradiation.