心钠素对培养人心包间皮细胞内钙离子浓度的影响 |
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引用本文: | 温绍君,王佐广,赵莉敏,高宇翔,文杰,张忱,徐燕玲,何士大. 心钠素对培养人心包间皮细胞内钙离子浓度的影响[J]. 解剖学报, 2002, 33(5): 501-504 |
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作者姓名: | 温绍君 王佐广 赵莉敏 高宇翔 文杰 张忱 徐燕玲 何士大 |
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作者单位: | 1. 北京安贞医院北京市心肺血管疾病研究所高血压研究室,北京,100029 2. 北京宣武医院,北京,100053 |
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摘 要: | 目的 探讨心纳素与人类心包间皮细胞之间的关系。 方法 分离人心包间皮细胞进行体外培养 ,用Fluo 3作为钙的指示剂 ,利用激光共聚焦显微镜测定 10 - 9mol L ,10 - 1 0 mol L ,10 - 1 1 mol L心钠素 (ANP)分别用作用于人心包间皮细胞后细胞内Ca2 (Ca2 )浓度变化。 结果 人心包间皮细胞存在细胞内Ca2 浓度的改变 ,随着ANP浓度的增加 ,细胞内Ca2 浓度出现非常明显的变化 (P <0 0 0 0 1) ;细胞内Ca2 浓度随时间变化也出现明显的变化 (P <0 0 0 0 1) ;不同时间测定的钙离子浓度与ANP浓度之间存在着交互作用 (P <0 0 0 0 1)。 结论 人心包间皮细胞存在细胞内Ca2 的改变 ;不同浓度的ANP作用于间皮细胞后引起细胞内Ca2 浓度出现明显不同的改变
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关 键 词: | 激光共聚焦显微镜 心包间皮细胞 钙离子 心钠素 |
EFFECT OF DIFFERENT KINDS OF ANP CONCENTRATION ON INTRACELLULAR CALCIUM LEVEL IN CULTURED HUMAN MESOTHELIAL CELLS |
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Abstract: | Objective To investigate the Ca 2 changes in the pericardium mesothlial cells by confocal laser scanning microscopy(CLSM) and uncover the relationship between the ANP and the pericardium mesothelial cells. Methods Using Fluo 3 as a marker, Ca 2 in the cultured mesothelial cells were determined at one second interval by CLSM after 10 -9 ?mol/L,10 -10 ?mol/L,10 -11 ?mol/L atrial uretic peptide(ANP) being added, respectively. Results Calcium concentration in the mesothelial cells all was high after the three kinds of ANP were added. The calcium concentration of ANP 10 -9 ?mol/L cells elevated suddenly(about 10 times of the normal level, P <0 0001) and soon lowered beyond the normal level when the ANP appeared, the calcium concentration of ANP 10 -10 ?mol/L cells first had a light decreasing and then increased to 6 times higher than the normal level in the plasma ( P <0 0001), while the ANP 10 -11 ?mol/L group cells just displayed a 4 times increase and then continued ( P <0 0001). Conclusion Calciumion in cultured human mesothelial cells were changed after the stimulation of different kinds of ANP concentration. Different ANP concentrations could introduce different degree of calcium influx. |
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Keywords: | CLSM Mesothelial cells Ca 2 ANP |
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