Intracellular translocation of glutathione S-transferase pi during oligodendrocyte differentiation in adult rat cerebral cortex in vivo |
| |
Authors: | Tamura Y Kataoka Y Cui Y Takamori Y Watanabe Y Yamada H |
| |
Affiliation: | Department of Anatomy and Cell Science, KMU 21C COE Project, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan. |
| |
Abstract: | Glutathione S-transferase (GST)-pi is a cytosolic isoenzyme used as a marker for mature oligodendrocytes in the mammalian brain. However, the cellular properties of GST-pi-immunoreactive [GST-pi (+)] cells in adult brain are not completely understood. We immunohistochemically demonstrated the existence of two subtypes of GST-pi (+) cells in the cerebral cortex of adult rats: one subtype exhibited GST-pi in the cytoplasm (C-type cells), while the other did mainly in the nucleus (N-type cells). The GST-pi (+) C-type cells were also immunopositive for 2',3'-cyclic nucleotide 3'-phosphodiesterase and RIP, indicating that they were mature oligodendrocytes, while the GST-pi (+) N-type cells expressed NG2, indicating that they were oligodendrocyte progenitor cells. Furthermore, observation of the fate of newly-generated cells by 5-bromodeoxyuridine-labeling revealed that the GST-pi (+) N-type cells differentiated into C-type cells. These findings indicate translocation of GST-pi from the nucleus to the cytoplasm during oligodendrocyte maturation. |
| |
Keywords: | oligodendrocyte progenitor cells NG2 CNPase RIP BrdU |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|