Characterization of Gag and Nef-specific ELISpot-based CTL responses in HIV-1 infected Indian individuals |
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Authors: | Mendiratta Sanjay Vajpayee Madhu Malhotra Uma Kaushik Shweta Dar Lalit Mojumdar Kamalika Chauhan Neeraj Kumar Sreenivas Vishnubhatla |
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Institution: | (1) HIV and Immunology Division, Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi, 110029, India;(2) Department of Epidemiology and Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA, USA;(3) Department of Medicine, University of California, San Francisco, CA, USA;(4) Department of Biostatistics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India |
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Abstract: | Cytotoxic T lymphocyte (CTL) responses to Gag have been most frequently linked to control of viremia whereas CTL responses
to Nef have direct relationship with viral load. IFN-γ ELISpot assay was used to screen CTL responses at single peptide level
directed at HIV-1 subtype C Gag and Nef proteins in 30 antiretroviral therapy naive HIV-1 infected Indian individuals. PBMCs
from 73.3% and 90% of the study population showed response to Gag and Nef antigens, respectively. The magnitude of Gag-specific
CTL responses was inversely correlated with plasma viral load (r = −0.45, P = 0.001), whereas magnitude of Nef-specific responses was directly correlated (r = 0.115). Thirteen immunodominant regions (6 in Gag, 7 in Nef) were identified in the current study. The identification of
Gag and Nef-specific responses across HIV-1 infected Indian population and targeting epitopes from multiple immunodominant
regions may provide useful insight into the designing of new immunotherapy and vaccines. |
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Keywords: | HIV-1 specific ELISpot Gag Nef CTL responses India |
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