DON对体外培养胃癌细胞SGC-7901、BGC-823凋亡的影响

目的探讨脱氧雪腐镰刀菌烯醇(DON)对胃癌细胞SGC-7901、BGC-823凋亡的影响及其可能机制。方法体外培养的胃癌细胞株SGC-7901、BGC-823细胞经100、500、1000μg/LDON处理72h,以流式细胞术(FCM)检测细胞凋亡情况及其量效关系,流式细胞术、免疫细胞化学和Westernblot检测凋亡相关蛋白Bax、Bcl-2蛋白的表达情况。结果FCM定量检测结果表明,DON各处理组细胞凋亡率均高于对照组,且均随DON浓度升高凋亡率相应增加,具有明显量效关系(SGC-7901:r=0.660,P<0.05,n=4;BGC-823:r=0.750,P<0.01,n=4)。FCM、免疫细胞化学和Westernblot结果显示,DON处理后SGC-7901、BGC-823细胞Bax蛋白表达上调,Bcl-2蛋白表达下降。结论DON可剂量依赖地诱导体外培养的胃癌细胞SGC-7901、BGC-823凋亡,其机制可能与上调Bax蛋白的表达,下调Bcl-2蛋白的表达有关。

Effects of deoxynivalenol on apoptosis of human gastric carcinoma cell line SGC-7901, BGC-823 in vitro

Objectlve To explore the putative effects and possible mechanisms of deoxynivalenol (DON) on the apoptosis of human gastric carcinoma cell line SGC-7901, BGC-823 in vitro. Methods SGC-7901 and BGC-823 ceils were treated with DON (50, 100, 1000tμg/L) for 72 hours, and then cells were harvested for the studies of apoptosis and the expression of Bax and Bel-2 with flow cytometry (FCM), immunocytochemical staining and Western blot. Results FCM results showed that the apoptosis rates of SGC-7901 and BGC-823 cells in DON treatment groups were higher than that in control, and positive dose-effect correlations could be found in both cell lines (SGC-7901 : r = 0.660, P < 0.05, n = 4, BGC-823: r = 0.750, P < 0.01, n = 4). FCM, immunocytochemical staining and Western blot results showed that the expression of Bax was increased while that of Bcl-2 was decreased in DON treatment groups of SGC-7901 and BGC-823. Conclusion The results suggested that DON could induce apoptosis of SGC-7901, BGC-823 cells in vitro in a dose-dependent manner, and possible mechanisms may be increased formation of Bax-Bax homology dimer and decreased formation of Bax-Bcl-2 dimer by up-regulation of the expression of Bax and down-regulation of that of Bcl-2.