质控品结果在控后再检测临床样本的可行性探讨

目的　探讨生化室内质控品结果在控后再检测临床样本工作程序的可行性。方法　自制了一批混合血清 ,分三组在 BECKMAN LX2 0型生化分析仪上检测 BUN等 1 0项常规生化项目。第一组在检测室内质控品结果在控后 ,临床样本上机前检测 ;第二组随机插在当天上午临床样本中检测 ;第三组随机插在当天下午的临床样本中检测。每组每次检测 2次 ,检测 40 d。每组取检测有效 (除去离群点 )的结果的均值进行单因素方差分析 ,比较各组间差异。结果　除 TC( P>0 .0 5 )外 ,其它 9个项目均无统计学差异 ( P<0 .0 5 )。结论　临床样本上机前先完成室内质控再检测临床样本工作程序具有可行性 ,但实验室要具备仪器状态好 ,试剂稳定等条件 ,工作人员要具有全程质量控制的素质。

The Feasible Study on Determining the Clinical Samples after All Controls under Control

Objective To observe the feasibility of the working procedure for beginning to measure the clinical samples after all controls are under control.Methods A set of the mixed serum made by ourselves was divided into three groups to run the routine biochemical items such as BUN by SYNCHRON clinical system LX20(BECKMAN).The first group of the serum was run before beginning the clinical samples after all controls were under control,the second group was inserted at random into the samples in the morning and the third group was inserted at random into the samples in the afternoon.Each group of the mixed serum was run repeatedly for 40 days and took the mean values of the valid results except the outlines to do one factor analysis to compare the difference with each other among the three groups.Results There were not statistical significance among the 9 items (P>0.05) except TC(TC,P<0.05).Conclusion It is a feasible working procedure to run the clinical samples after all controls are under control.But the laboratory must have the instruments with good conditions,the stable reagents and the experienced stuffs with the whole quality control.