集聚蛋白在免疫细胞中的表达及其意义

为研究在神经突触中发挥重要作用的突触调节蛋白集聚蛋白是否在免疫细胞中表达并影响淋巴细胞的活化。首先通过RT-PCR、荧光免疫组织化学和FACS分别从mRNA水平和蛋白水平观察集聚蛋白在各种免疫器官、细胞中的表达;通过实时定量PCR检测分析集聚蛋白的表达与淋巴细胞活化的关系;并构建针对集聚蛋白的反义质粒,从而进一步研究下调集聚蛋白的表达对淋巴细胞活化的影响。结果发现集聚蛋白广泛表达于脾脏、胸腺、淋巴结等多种免疫器官中并在T细胞、B细胞、imDC、mDC及巨噬细胞中均有表达,其表达含量与淋巴细胞的活化状态密切相关,且其表达被下调后淋巴细胞的活化显著被抑制。因而组成性表达于各种免疫细胞中的集聚蛋白可被诱导性上调,并参与了淋巴细胞的活化。

The expression and implication of agrin in immunocytes

To study whether agrin, a synaptic regulated protein in neurological synapse, is expressed in immuno-cytes and affects the proliferation of lymphocyte. RT-PCR, immunofluorescence histochemistry and FACS were used to analyze the expression of agrin in some immune organs and primary immunocytes. Then real time RT-PCR and 3H-TdR incorporation assay were performed to evaluate the relationship between the expression of agrin and statue of lymphocyte activation. Constructed antisense plasmids were used to explore function of agrin in the proliferation of lymphocyte activation. Our experimental results demonstrated that agrin were constitutively expressed in various immune organs such as spleen, thymus and lymphonode, immunocytes including CD4+, CD8+ T cells, B220+ B cells, imDC, mDC, and MΦ. There was significant relationship between the expression of agrin and activation of lymphocytes. The proliferation of lymphocytes can be inhibited after the agrin expression being down regulated. These results suggest that constitutively expressed agrin in immunocytes participates in the proliferation of lymphocytes.