RP-HPLC法测定莽吉柿果皮中3个氧杂蒽酮类化合物的含量

目的:建立一种莽吉柿果皮中3个氧杂蒽酮类化合物的RP-HPLC含量测定方法。方法:色谱柱为Waters XTerra RP18色谱柱(150 mm×4.6 mm,5μm),以水-0.5%醋酸(A)、甲醇-0.5%乙酸(B)为流动相,梯度洗脱,流速为1.0 mL·min-1,检测波长为317 nm,柱温为30℃。结果:3-异倒捻子素、α-倒捻子素、β-倒捻子素浓度分别在21.3～213,107.7～1077,10.6～106μg·mL-1范围内线性关系良好(r≥0.9991);平均回收率分别为102.6%,98.5%,103.5%,RSD分别为1.7%,1.2%,2.0%(n=6)。结论:所建立的方法便捷、准确,重复性好,可以为莽吉柿果皮的质量控制提供一定的参考。

RP-HPLC determination of 3 xanthones content in mangosteen fruit skin

Objective:To establish an HPLC method of the determination of 3 xanthones content in mangosteen fruit skin.Methods:The content of 3 xanthones were determined by Waters XTerra RP18(150 mm×4.6 mm,5 μm) column,the mobile phase consisted of water-0.5% acetic acid(A) and methanol-0.5% acetic acid(B) with gradient elution at the flow rate of 1.0 mL·min-1,the detection wavelength was 317 nm,and the temperature of column was 30 ℃.Results:The calibration curves of 3-isomangostin,α-mangostin,β-mangostin were linear in the ranges of 21.3-213,107.7-1077,10.6-106 μg·mL-1(r ≥ 0.9991),respectively.The average recoveries of 3 xanthones were 102.6%,98.5%,103.5% and RSDs(n=6) were 1.7%,1.2%,2.0%,respectively.Conclusion:The method is easy,sensitive,specific,and accurate with good reproducibility.It can be used for quality control of mangosteen fruit skin.