白介素-9基因多态性及其血清水平与黑衣壮儿童哮喘发病的关系

目的：探讨白介素-9（IL-9）基因位点rs2069882和rs2069885多态性和血清IL-9水平与黑衣壮儿童哮喘发病关系。方法选择黑衣壮儿童，其中哮喘缓解期80例，健康组100例。采用聚合酶链反应－连接酶检测反应（ PCR-LDR）方法检测IL-9的rs2069882和rs2069885位点多态性，采用ELISA法检测血清IL-9水平。分析两组间IL-9基因型和等位基因分布情况及血清水平。结果 IL-9基因 rs2069885位点基因分型只检测出CC基因型；rs2069882位点基因分型仅检出AA、AG基因型，而且哮喘组与对照组的AA、AG基因型及等位基因分布比较，差异均无统计学意义（P＞0．05）。健康组血清IL-9水平明显低于哮喘组（P＜0．05）。结论 IL-9基因位点rs2069882的多态性可能不是黑衣壮儿童哮喘的易感因素，而血清IL-9水平升高可能与哮喘发病有关联。

Association of gene polymorphisms and serum level of interleukin-9 with asthma in black-clothes Zhuang ethnic children

Objective To explore the association of gene polymorphisms of rs2069882 and rs2069885 loci and serum level of interleukin-9 (IL-9) with asthma in black-clothes Zhuang ethnic children .Methods Eighty children with asthma in remission and 100 healthy controls were enrolled in total .Gene polymorphisms of rs 2069882 and rs2069885 loci of IL-9 gene were detected by polymerase chain reaction-ligase detection reaction,while serum IL-9 level was measured by enzyme linked immunosorbent assay .Genotype,allele distribution,and serum level of IL-9 in both groups were analyzed.Results Only CC genotype was detected on rs2069885 locus of IL-9.As for the rs2069882 locus,only CC and AA genotype was detected ,but no significant difference was observed in AA and AG genotype or allele distribution between the two groups(P>0.05).Serum level of IL-9 was lower in the healthy group than in the asthma group(P<0.05).Conclusion Gene polymorphisms rs2069882 locus of IL-9 might not the risk factor for asthma in black-clothes Zhuang ethnic children,however,the increased serum IL-9 might be associated with the occurrence of asthma.