| 复制缺陷型腺病毒Ad-p14ARF治疗肝细胞癌的实验研究及作用机制的探讨 |
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| 引用本文： | Haili Qian Haifeng Song Xueyan Zhang Xiao Liang Ming Fu Chen Lin. 复制缺陷型腺病毒Ad-p14ARF治疗肝细胞癌的实验研究及作用机制的探讨[J]. 中德临床肿瘤学杂志, 2007, 6(1): 22-26. DOI: 10.1007/s10330-006-0025-9 |
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| 作者姓名： | Haili Qian Haifeng Song Xueyan Zhang Xiao Liang Ming Fu Chen Lin |
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| 作者单位： | [1]State Key Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, PUMC, Beijing 100021, China [2]State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | Objective： To study the therapeutic effect and mechanisms of recombinant adenovirus Ad-p14ARF in hepatocellular carcinoma cell lines. Methods： Morphology and trypan blue assay were adopted to evaluate the proliferation of different liver cancer cells after Ad-p14ARF infection. Cell apoptosis was confirmed by detecting phosphatidylserine （PS） externalization with Annexin V/PI double staining. Western blotting assay analyzed the expression of related proteins. Subcutaneous tumor model of BEL7402 was established to evaluate the therapeutic ability of Ad-p14ARF. Results： Ad-p14ARF suppressed cell growth, proliferation and promoted cell apoptosis of cancer cell lines with different genetic background. Ad-p14ARF inhibited growth of liver cancer cells （HepG2, BEL7402） in a dose-dependent manner. Ad-p14ARF leaded to overexpression of Bax and p21, which were the downstream regulating genes of p53. Ad-p14ARF suppressed tumor growth significantly in the experimental therapy in nude mice bearing subcutaneous tumor of BEL7402. Conclusion： P14ARF gene is a powerful tumor suppressor gene to be used in cancer gene therapy. It may play an important role in gene therapy against the malignancies in the future.
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| 关 键 词： | 肝细胞癌 基因治疗 机理 重组腺病毒 Ad-p14ARF |
| 收稿时间： | 2006-11-21 |
| 修稿时间： | 2006-12-10 |
The therapy and mechanisms of replication-deficient recombinant adenovirus Ad-p14ARF in hepatocellular carcinoma |
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| Haili Qian,Haifeng Song,Xueyan Zhang,Xiao Liang,Ming Fu,Chen Lin. The therapy and mechanisms of replication-deficient recombinant adenovirus Ad-p14ARF in hepatocellular carcinoma[J]. The Chinese-German Journal of Clinical Oncology, 2007, 6(1): 22-26. DOI: 10.1007/s10330-006-0025-9 |
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| Authors: | Haili Qian Haifeng Song Xueyan Zhang Xiao Liang Ming Fu Chen Lin |
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| Affiliation: | (1) State Key Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, PUMC, Beijing, 100021, China;(2) State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, Guangzhou, 510060, China |
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| Abstract: | Objective: To study the therapeutic effect and mechanisms of recombinant adenovirus Ad-p14ARF in hepatocellular carcinoma cell lines. Methods: Morphology and trypan blue assay were adopted to evaluate the proliferation of different liver cancer cells after Ad-p14ARF infection. Cell apoptosis was confirmed by detecting phosphatidylserine (PS) externalization with Annexin V/PI double staining. Western blotting assay analyzed the expression of related proteins. Subcutaneous tumor model of BEL7402 was established to evaluate the therapeutic ability of Ad-p14ARF. Results: Ad-p14ARF suppressed cell growth, proliferation and promoted cell apoptosis of cancer cell lines with different genetic background. Ad-p14ARF inhibited growth of liver cancer cells (HepG2, BEL7402) in a dose-dependent manner. Ad-p14ARF leaded to overexpression of Bax and p21, which were the downstream regulating genes of p53. Ad-p14ARF suppressed tumor growth significantly in the experimental therapy in nude mice bearing subcutaneous tumor of BEL7402. Conclusion: P14ARF gene is a powerful tumor suppressor gene to be used in cancer gene therapy. It may play an important role in gene therapy against the malignancies in the future. |
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| Keywords: | recombinant adenovirus p14ARF liver cancer cells gene therapy |
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