| 抗羊垂体催乳素单克隆抗体性质研究 |
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| 引用本文： | 周柏光,杨翰仪,麦荫乔. 抗羊垂体催乳素单克隆抗体性质研究[J]. 吉林大学学报(医学版), 1991, 0(4) |
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| 作者姓名： | 周柏光 杨翰仪 麦荫乔 |
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| 作者单位： | 北京第一传染病医院病毒研究室(周柏光)，白求恩医科大学生化教研室(杨翰仪)，白求恩医科大学生化教研室(麦荫乔) |
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| 摘 要： | 本文报道了应用淋巴细胞杂交瘤技术将羊垂体催乳素(oPRL)免疫过的BALB/c小鼠脾细胞与NS-O骨髓瘤细胞融合。制备了5株分泌抗oPRL单克隆抗体的杂交瘤细胞株。其中1种单克隆抗体属IgG1,4种属IgG2a。ELISA检测小鼠腹水抗体稀释度均在5×10~4倍以上。用固相夹心免疫放射测定法测出5种单克隆抗体可识别oPRL分子上3个不同的抗原决定簇,本文还对5种单克隆抗体与和oPRL结构相似的生长激素进行交义反应以鉴别其特异性。
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| 关 键 词： | 羊催乳素 单克隆抗体 抗原决定簇 特异性 |
STUDY ON PROPERTIES OF MONOCLONAL ANTIBODIES TO OVINE PROLACTIN |
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| Zhou Boguang,Yang Hanyi,Mai Yinqiao. STUDY ON PROPERTIES OF MONOCLONAL ANTIBODIES TO OVINE PROLACTIN[J]. Journal of Jilin University: Med Ed, 1991, 0(4) |
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| Authors: | Zhou Boguang Yang Hanyi Mai Yinqiao |
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| Affiliation: | Department of Biochemistry |
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| Abstract: | Eive monoclonal antibodies (McAb) against prolactin(oPRL)were obtained by lymphocyte hybridoma technique. One of the McAbs was IgG1, and the others were IgG2a. Three different antigenic determinants on oPRl could be recognized by these McAbs, using two-site sandwish radioimmunoassay. The cross reactions of McAb to ovine growth hormone was performed to establish the specificity of each McAb. |
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| Keywords: | Ovine prolactin Monoclonal antibody Antigenic determinant Specificity |
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