锂-匹鲁卡品诱导大鼠癫痫发作后海马齿状回IL-1 mRNA表达的变化

目的:观察大鼠癫痫发作后海马齿状回细胞因子IL-1(IL-1R、IL-1β、IL-1ra)mRNA的表达变化,探讨IL-1在癫痫发作中的作用.方法:大鼠随机分为对照组和实验组.实验组大鼠腹腔注射氯化锂以及匹鲁卡品,对照组注射生理盐水,观察其行为学特征,并用RT-PCR方法检测大鼠海马齿状回内细胞因子IL-1 mRNA的动态表达变化.结果:大鼠腹腔注射锂-匹鲁卡品后30 min内相继出现严重癫痫持续状态发作,实验组各种细胞因子在严重癫痫持续发作后1 h表达水平开始明显增加(P<0.05),随着时间的延长表达逐渐增多,IL-1β的表达于发作后6 h达高峰,IL-1ra和IL-1 R1则在发作后12 h达高峰,随后表达逐渐下降,各因子到发作后48 h表达降低但仍高于对照组表达水平(P<0.05).结论:癫痫发作后急性期细胞因子IL-1β、IL-1ra及其受体IL-1 R1在不同时间点均有增加,提示炎性因子参与了癫痫发作以及癫痫发作后脑损伤.

Expression of IL-1 mRNA in the dentate gyrus of adult rats following lithium-pilocarione-induced seizures

AIM:To examine the expression of IL-1β,IL-1 ra and IL-1R mRNA in the dentate gyrus of adult rats after lithium-pilocarpine(Li-PILO)-induced seizures.METHODS:Seizure epilepticus(SE)was induced using Li-PILO intraperitoneally(i.p.)injection.Lithium chloride was injected 18-20 h prior to PILO.The rats in the control group were given physiological saline instead of pilocarine.We observed the behavior of rats and examined the expression of cytokines mRNA in dentate gyrus by RT-PCR.RESULTS:Rats had seizure epilepticus in 30 min after administrated of pilocarpine.Cytokines(IL-1β,IL-1 ra and IL-1R)began to increase significantly(P<0.05)at 1h after SE,and the peak was at 6-12 h.Expression of all cytokines declined on 48 h after SE.However these cytokines still kept higher than the control(P<0.05).CONCLUSION:Our findings suggestted that SE lead to increased expression of IL-1,IL-1 re and IL-1 R1 after lithium-pilocarpine-induced seizures.Indicating that cytokines involved in SE and brain impairment after seizure.