An improved plasma clot technique for initiating mammalian fibroblast cultures

Summary A modified plasma clot technique is described, which has been used with a high degree of success in initiating fibroblast cultures from tissues of human, cat, and dog origin. The technique offered real advantages in terms of production of healthy rapidly growing cultures, although human skin biopsies proved to take significantly longer to explant than cat or dog biopsies. There was no difference in explant time when cat biopsies were of skin or connective tissue origin. The skin from a series of insulin dependent (juvenile onset) diabetics explanted as rapidly as a series of normal biopsies.