毛金竹叶提取物化学成分的分离与鉴定

目的分离、鉴定毛金竹(Phyllostachys nigra(Lodd.exLindl.)Munro var.henonis(Mitf.)Stepfex Rendle)竹叶提取物的化学成分。方法采用反复硅胶柱色谱、Sephadex LH-20柱色谱、开放ODS柱色谱、制备薄层色谱等方法进行分离纯化,并通过理化性质和光谱数据鉴定其化学结构。结果从其乙酸乙酯萃取层和正丁醇萃取层分离得到12个化合物,分别鉴定为β-谷甾醇(β-sitosterol,1)、苜蓿素(tricin,2)、胡萝卜苷(daucosterol,3)、丁二酸(butanedioic acid,4)、胸腺嘧啶(5-methylu-racil,5)、苜蓿素-7-O-β-D-葡萄糖苷(tricin-7-O-β-D-glucopyranoside,6)、牡荆苷(vitexin,7)、苜蓿素-7-O-新橙皮糖苷(tricin-7-O-neohesperidoside,8)、荭草苷(orientin,9)、异荭草苷(isoorientin,10)、尿嘧啶(uracil,11)、胸腺嘧啶脱氧核苷(thymidine,12)。结论化合物4~6、8、11、12为首次从该属植物中分离得到。

Chemical constituents of the extraction of bamboo leaves from Phyllostachys nigra (Lodd.ex Lindl.) Munro var. henonis (Mitf.) Stepf ex Rendle

Objective To study the chemical constituents of the extraction of bamboo leaves of Phyllostachys nigra (Lodd.ex Lindl.) Munro var. henonis (Mitf.) Stepf. ex Rendle. Methods The compounds were isolated by chromatography on silica gel column, Sephadex LH-20, open ODS column and identified on the basis of physico-chemical constants and spectral analysis. Result Twelve compounds were isolated from EtOAc soluble fraction and n-BuOH soluble fraction of this plant and identified as β-sitosterol (1), tricin (2), daucosterol (3), butanedioic acid (4), 5-methyluracil (5), tricin-7-O-β-D-glucopyranoside (6), vitexin (7), tricin-7-O-neohesperidoside (8), orientin (9), isoorientin(10), uracil(11), thymidine (12). Conclusion Compounds 4, 5, 6, 8, 11, 12 are obtained from this genus for the first time.