| 血红素加氧酶-1抑制香烟烟雾提取物致体外人肺A549细胞黏液高分泌 |
| |
| 引用本文： | 雷蕊霞,周向东. 血红素加氧酶-1抑制香烟烟雾提取物致体外人肺A549细胞黏液高分泌[J]. 基础医学与临床, 2010, 30(3): 246-251 |
| |
| 作者姓名： | 雷蕊霞 周向东 |
| |
| 作者单位： | 重庆医科大学,附属第二医院,呼吸内科,重庆,400010 |
| |
| 基金项目： | 国家自然科学基金(30770951) |
| |
| 摘 要： | 目的探讨血红素加氧酶-1(HO-1)对香烟烟雾提取物(CSE)所致人气道黏液高分泌的影响。方法用CSE刺激A549细胞,复制黏液高分泌细胞模型。分为对照组、CSE组、氯化高铁血红素(Hemin)组和锌原卟啉(ZnPPIX)组,观察黏蛋白(MUC)5AC、表皮生长因子受体(EGFR)、磷酸化(p)-EGFR、HO-1及双功能氧化酶1(Duox1)的表达。用MTT法测定细胞活性;RT-PCR法检测HO-1、Duox1、EGFR及MUC5AC的mRNA;Western blot法检测p-EGFR、EGFR、Duox1和HO-1蛋白;ELISA法检测细胞裂解液中MUC5AC蛋白表达。结果CSE组MUC5AC的mRNA吸光度积分相对值和蛋白水平分别为0.660±0.044和(157±3)μg/mg,较对照组0.412±0.043和(105±8)μg/mg明显升高(P<0.05),p-EGFR蛋白水平、EGFR、HO-1及Duox1的mRNA和蛋白水平也较对照组明显增高。与CSE组相比,Hemin组HO-1mRNA及蛋白进一步增高,而p-EGFR蛋白水平、Duox1、EGFR及MUC5AC的mRNA和蛋白水平明显回降。与...
|
| 关 键 词： | 黏蛋白类 香烟烟雾 受体 表皮生长因子 血红素加氧酶-1 |
| 收稿时间： | 2009-07-27 |
| 修稿时间： | 2009-10-10 |
Heme oxygenase-1 downregulates mucous hypersecretion induced by cigarette smoke in cultured A549 cells |
| |
| LEI Rui-xia,ZHOU Xiang-dong. Heme oxygenase-1 downregulates mucous hypersecretion induced by cigarette smoke in cultured A549 cells[J]. Basic Medical Sciences and Clinics, 2010, 30(3): 246-251 |
| |
| Authors: | LEI Rui-xia ZHOU Xiang-dong |
| |
| Affiliation: | Dept.of Respiratory Medicine;the Second Affiliated Hospital;Chongqing Medical University;Chongqing 400010;China |
| |
| Abstract: | Objective To investigate the effects of heme oxygenase-1(HO-1) on the signal transduction pathway of mucin(MUC) expression induced by cigarette smoke.Methods The cell model of mucous hypersecretion was made by human lung A549 cell stimulated by cigarette smoke extract(CSE),the cells were divided into 4 groups:negative control group,CSE treatment group,hemin pre-treatment group and ZnPPIX pre-treatment group.The expression of MUC5AC,epidermal growth factor receptor(EGFR),p-EGFR,HO-1 and dual oxidase 1(Duox1)... |
| |
| Keywords: | mucins cigarette smoke receptor epidermal growth factor heme oxygenase-1 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《基础医学与临床》浏览原始摘要信息 |
|
点击此处可从《基础医学与临床》下载全文 |
